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14879 Sigma

BIS-TRIS

BioUltra, ≥99.0% (NT)

Synonym: 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane

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Properties

Related Categories Alphabetical, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers
InChI Key   OWMVSZAMULFTJU-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.0% (NT)
form   powder
impurities   insoluble matter, passes filter test
ign. residue   ≤0.3%
loss   ≤0.5% loss on drying, 20 °C (HV)
pH   9.5-11.0 (25 °C, 1 M in H2O)
useful pH range   5.8 - 7.2
pKa (25 °C)   6.5
solubility   H2O: soluble1 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
absorption   absorption1M/270 nm, H2O cut-off
λ   1 M in H2O
UV absorption   λ: 280 nm Amax: 0.15

Description

Other Notes

Effective buffer for the assay of creatine kinase; Effectively protects hemoglobin during freeze-drying; Catholyte in IEF of 2-D gel electrophoresis.

Easily compare specifications for BIS-TRIS products with the BIS-TRIS specification table.

Application

BIS-TRIS can be used to study biological buffers. BIS-TRIS has been used in a study that synthesized ferritin grafted with temperature-responsive poly(N-isopropylacrylamide) (PIPAAm-ferritin) by a coupling reaction using PIPAAm and ferritin for obtaining stimuli-responsive biomaterials. BIS-TRIS has also been used in a study that synthesized and described the photochemical properties of oligo- ortho –azobenzenes.

Price and Availability


Interactive Buffer Table
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Peer-Reviewed Papers
15

References

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