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15361 Sigma

Bleomycin sulfate from Streptomyces verticillus

for fluorescence, mixture of bleomycin sulfate salts, lyophilized, powder, white

Synonym: Blenoxane, Bleo, Blexane

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Properties

Related Categories Antibiotics, Antibiotics A to Z, Antibiotics A-F, Antibiotics by Application, Antifungal,
biological source   from Streptomyces verticillus
grade   for fluorescence
InChI Key   OOXTWFJZZAJGKA-CNLAFNBISA-N
form   powder
quality   lyophilized
color   white
solubility   H2O: soluble20 mg/mL, clear, colorless
cation traces   Cu: ≤1000 mg/kg
Mode of action   DNA synthesis | interferes
  DNA synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
  neoplastics
storage temp.   2-8°C

Description

Components

This product is a mixture of glycopeptides antibiotics containing Bleomycin A2 (70%) and B2(30%). Bleomycins differ from each other in the terminal amine and show varying biological activity.

Biochem/physiol Actions

Bleomycin sulfate binds to DNA, causes ssDNA scission at specific base sequences and inhibits DNA synthesis. This inhibitory action requires bleomycin to bind oxygen and a metal ion. It can also cleave RNA, to a lesser degree but more selectively. It acts as an inducer and regulator of apoptosis and inhibits tumor angiogenesis.

Caution

This product should be stored at 2-8°C.

Packaging

Packaged in a bottomless glass bottle with its contents inside an inserted fused cone.

Preparation Note

The product is soluble in water in water at 20 mg/mL and is active for several days. A solution of 2 units/mL in 0.1 M neutral potassium phosphate should be used within 14 days. A solution is active for three months at 3 units/mL in normal saline.

Application

Bleomycin sulfate is used to study tumor-specific cytotoxic activity in certain cell lines such as human normal oral cells(gingival fibrobast HGF, pulp cell HPC and periodontal ligament fibroblast HPLF), human oral squamous cell carcinoma (HSC-2, HSC-3, HSC-4, Ca9-22 and NA) and human promyelocytic leukemia HL-60 cells. It is used to study free radical-mediated mechanisms of DNA strand scission/breaking and as a transformed cell selection agent, especially for plant transformants. Recommended for use as a selection agent at 10-100 μg/ml.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

Price and Availability


Laboratory Gloves
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
EC5991990
Protocols & Articles
Peer-Reviewed Papers
15

References

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