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16016 Sigma

BRILA MUG Broth

for microbiology

Purchase

Properties

Related Categories A - M, All Liquid Media/Broths, Citrobacter, Escherichia coli & Coliforms, Fluorogenic Media,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   brilliant green, 0.133 g/L
  lactose, 10 g/L
  4-methylumbelliferyl-β-D-glucuronide, 0.1 g/L
  ox-bile (dried), 20 g/L
  peptone, 10 g/L
  L-tryptophan, 1 g/L
final pH   7.2±0.2 (25 °C)
suitability   selective and differential for Citrobacter
  selective and differential for Escherichia coli and Coliforms
  selective and differential for Lactobacillus
  selective and differential for Staphylococcus

Description

Application

In the detection of E. coli and coliforms. Bile and brilliant green extensively inhibit the growth of accompanying flora, in particular gram-positive microorganisms. The presence of E. coli results in fluorescence in the UV. A positive indole test and possibly gas formation from lactose fermentation provide confirmation.
The broth can be used in conjunction with the MPN method for E. coli and coliform enumeration in the water of bathing areas.

Other Notes

Screening of bathing-water for coliforms according to EC directive

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Selective growth media for differentiation and detection of Escherichia coli and other coliforms

Normal constituents of the intestinal flora of animals, coliforms are rod-shaped, Gram-negative, non-spore forming facultative anaerobes. They ferment lactose with the production of acid and gas when...
Jvo Siegrist
AnalytiX Volume 8 Article 4
Keywords: Biochemistry, Clinical, Culture media, Electron capture detector, Fermentation, Immunology, Indicators, Microbiology, Molecular biology

Peer-Reviewed Papers
15

References

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