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16630 Sigma

Atto 680-Streptavidin

BioReagent, suitable for fluorescence

Synonym: Streptavidin-Atto 680

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Properties

Related Categories Biochemicals and Reagents, Fluorescent Conjugates, Fluorescent Probes, Labels, Particles and Stains, Streptavidin/Avidin Conjugates
product line   BioReagent
solubility   H2O: soluble (readily soluble)
fluorescence   λex 680 nm; λem 695 nm in 0.1 M phosphate pH 7.0
suitability   corresponds for gel electrophoresis
  suitable for fluorescence
storage temp.   −20°C

Description

General description

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Application

Atto 680 is a fluorescent label with high molecular absorption (125.0) and quantum yield (0.30), as well as a sufficient Stoke′s shift (excitation maximum 680 nm, emission maximum 700 nm). It has limited cis-trans-isomerization, which enhances its brightness and reproducibility. Conjugation of Atto 680 to steptavidin does not significantly alter the label′s spectral data regarding excitation and emission maxima. Additionally, the molar absorbance and quantum yield remain high. Such conjugation is useful as a secondary detection reagent in histochemical applications, flow cytometry, microarrays, blot analysis, and immunoassays. Atto 680-streptavin does not show strong fluorescence until bound to biotin.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Fluorescent Labeling of Peptides

Labeling peptides with fluorescent dyes or other labels provides powerful tools for the investigation of biological relevant interactions like receptor-ligand-binding,1-3 protein structures,4-6 and e...
ChemFiles Volume 5 Article 12
Keywords: Alzheimer Disease, Biochemistry, Electrophoresis, Enzyme activity, Immobilization, Ligands, Methods, Microscopy, Peptide synthesis, Separation, Solid phase peptide synthesis, Solvents, Spectroscopy, Tools

Superior Fluorescent Imaging Using Atto Dyes

Activated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescen...
BioFiles 2011, 6.3, 5.
Keywords: Absorption, Applications, Bacterial conjugations, Confocal microscopy, Degradations, Enzyme Assays, Extinction coefficient, Flow cytometry, In Situ hybridization, Infrared spectroscopy, Isomerizations, Microarray Analysis, Microscopy, Molecular probes, Nucleic acid hybridization, Protocols, Semiconductor, Separation, Spectroscopy, Support

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