16805 Sigma

Atto 425 NHS ester

BioReagent, suitable for fluorescence

Synonym: Atto 425-N-hydroxysuccinimide ester



Related Categories Atto Labels, Biochemicals and Reagents, Fluorescent Labels, Fluorescent Probes, Labels, Particles and Stains, Luminescent Compounds/Detection,
product line   BioReagent
solubility   DMF: soluble
  DMSO: soluble
  H2O: soluble
fluorescence   λex 437 nm; λem 483 nm in 0.1 M phosphate pH 7.0
suitability   suitable for fluorescence
storage temp.   −20°C



Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Atto 425 NHS ester is used in the development of bifunctional fluorescent, inorganic micro-partices (Zeolite L crystals) designed for targeting or imaging mutliple components . Reacts with amino groups or amine modified oliognucleotides to form a stable amine bond. Utilized as an essential component of molecular beacons, which were designed for use in real-time PCR assays to allow for rapid and accurate identification of gram-negative bacteria . When conjugated with benzylguanine, the fluorescence of Atto 425-NHS is quenched but becomes fluorescent upon labeling of the SNAP-tag fusion protein, making this conjugate an ideal candidate for experiments in living cells . Used as one of the donor probes in three-color FRET experiments designed to investigate the kinetics of RNA-protein complex formation .

General description

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Other Notes

Study of stability during one- and two-photon excitation in fluorescence correlation spectroscopy, FCS; employed in triple-color coincidence analysis.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
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