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17783 Sigma

Calcein-AM

BioReagent, suitable for fluorescence, ≥96.0% (HPLC)

Synonym: Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester, Calcein acetoxymethyl ester

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Properties

Related Categories Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability, Biochemicals and Reagents, Calcium Indicators, Cell Culture, Cell Viability,
InChI Key   XKFSBWQWNMZWFA-UHFFFAOYSA-N
product line   BioReagent
assay   ≥96.0% (HPLC)
fluorescence   λex 496 nm; λem 516 nm in 0.1 M Tris pH 8.0, esterase; Ca2+
suitability   suitable for fluorescence
storage temp.   −20°C

Description

Frequently Asked Questions

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Application

Non-fluorescent cell permeable derivative of calcein, becomes fluorescent on hydrolysis. Used as a neutral substrate for multidrug resistance protein, MRP; employed in tumor research.

Calcein-AM has been used in flow cytometry.

General description

Liposomes contain a self-quenching fluorescent dye called calcein. It is a fluorescent dye with excitation and emission wavelengths of 495/515 nm, respectively. It self-quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage. The leakage rate is calculated on the basis of the fluorescence increment as the entrapped calcein is leaked out of the liposomal compartment. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

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BioFiles 2011, 6.3, 23.
Keywords: Applications, Biochemistry, Biological processes, Cancer, Cardiovascular, Central Nervous System, Diseases, Extinction coefficient, Indicators, Inflammation, Methods, Microscopy, Neurotransmission, Sol-gel, Type

Peer-Reviewed Papers
15

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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