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17937 Sigma

25-Hydroxyvitamin D2

≥98.0% (HPLC)

Synonym: 25-Hydroxyergocalciferol, 25-Hydroxycalciferol

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Properties

Related Categories Biochemicals and Reagents, Vitamin D, Vitamin D2, Vitamins
InChI Key   KJKIIUAXZGLUND-ICCVIKJNSA-N
Quality Level   PREMIUM
assay   ≥98.0% (HPLC)
storage temp.   −70°C

Description

Packaging

1 mg in poly bottle

Amber polypropylene vial

Biochem/physiol Actions

Ergocalciferol (vitamin D2) is activated in vivo by hydroxylation to 25-hydroxyergocalciferol and 1α,25-dihydroxycalciferol. Activated Vitamin D2 molecules may be used in a wide range of studies to assess their effects on functions such as immune function and calcium homeostasis.

Other Notes

Tandem Mass Spectrometry data independently generated by Scripps Center for Metabolomics is available to view or download in PDF. 17937.pdf Tested metabolites are featured on Scripps Center for Metabolomics METLIN Metabolite Database. To learn more, visit sigma.com/metlin.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2811 6.1 / PGII
WGK Germany 
3
Protocols & Articles

Articles

Analysis of Vitamin D in Milk and Infant Formula using UHPLC/MS/MS per AOAC Method 2011.11

Fortification of milk, dairy products, infant and adult nutritionals with vitamin D is required in many countries to provide additional dietary vitamin D. The fortification of milk in the USA, for ex...
Olga Shimelis
Reporter US Volume 33.2
Keywords: Chromatography, Evaporation, High performance liquid chromatography, Mass spectrometry, Metabolites, Sample preparations, Saponifications, Separation, Solvents, Vitamins

HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 20 °C

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 20 °C
Keywords: Chromatography, Clinical, High performance liquid chromatography, Vitamins

HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 30 °C

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 30 °C
Keywords: Chromatography, Clinical, High performance liquid chromatography, Vitamins

HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 40 °C

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 40 °C
Keywords: Chromatography, Clinical, High performance liquid chromatography, Vitamins

HPLC Analysis of 25-Hydroxyvitamin D3 and 25-Hydroxyvitamin D2 on Ascentis® Express F5

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of 25-Hydroxyvitamin D3 and 25-Hydroxyvitamin D2 on Ascentis® Express F5
Keywords: Chromatography, Clinical, High performance liquid chromatography, Vitamins

LC/MS Analysis of 25-Hydroxyvitam D3 and 25-Hydroxyvitamin D2 on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of 25-Hydroxyvitam D3 and 25-Hydroxyvitamin D2 on Ascentis® Express C18
Keywords: Chromatography, Clinical, High performance liquid chromatography, Vitamins

LC/MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 on Ascentis® Express F5

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 on Ascentis® Express F5
Keywords: Chromatography, Clinical, High performance liquid chromatography, Indicators, Liquid chromatography mass spectrometry, Mass spectrometry, Solvents, Vitamins

LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Solvents, Vitamins

LC/MS/MS of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3, and 3-epi-25-Hydroxyvitamin D3 in Serum or Plasma on Ascentis® Express F5 after SPE with HybridSPE®-Phospholipid, Minimization of Matrix Effects

From our library of Articles, Sigma-Aldrich presents LC/MS/MS of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3, and 3-epi-25-Hydroxyvitamin D3 in Serum or Plasma on Ascentis® Express F5 after SPE with HybridSPE®-Phospholipid, Minimization of Matrix Effects
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Sample preparations, Solid phase extractions, Vitamins

Vitamin D and Prevention of Human Disease

Vitamin D2 (ergocalciferol) is naturally synthesized from ergosterol by invertebrates, fungi, and plants in response to ultraviolet B irradiation, while vitamin D3 synthesis (cholecalciferol) is uniq...
Chloe McClanahan
BioFiles 2009, 4.6, 4.
Keywords: Absorption, Angiogenesis, Biofiles, Biological processes, Cancer, Clinical, Gene expression, Growth factors, Hormones, Hydroxylations, Ligands, Metabolites, Transcription, Type, Vitamins

Peer-Reviewed Papers
15

References

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