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17951 Sigma

Fluorescent IEF-Marker-Mix for CE and Gel Electrophoresis

Synonym: IEF-Marker-Mix fluorescent for CE and Gel Electrophoresis

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Description

Application

Suitable as IEF-Marker for HPCE and for IEF-gels

Other Notes

Mixture of 5 fluorescent IEF-Markers: pI 4.0, 5.5, 7.2. 7.6, 9.0 in 5 mM HCl:2-propanol (1:1), filtered through a 0.45 μm membrane filter.

Reconstitution

For use in HPCE the marker mix should be diluted 1:100 in a suitable ampholyte solution (e.g. 2% ampholyte 3-10), UV-detection at e.g. 280 nm (20 °C). The maximum absorbances of the individual markers are between 308 and 350 nm. For fluorescence detection use an excitation wavelength of 310 nm (individual excitation maxima: 310 to 360 nm); the emission maxima of the individual markers will lie between 412 and 440 nm. For use in IEF-gels the marker mix can be loaded directly onto the gel (max. 1 μl per lane). The fluorescent bands are visualized directly at the end of focusing by UV light illumination (254 or 300 nm). During gel staining (for protein visualization) the fluorescent bands are lost.

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2920 3(8) / PGII
WGK Germany 
1
Flash Point(F) 
73.4 °F
Flash Point(C) 
23 °C
Protocols & Articles
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02192 UV-VIS detectable IEF Marker, BioChemika, pI 7.7
73743 UV-VIS detectable IEF Marker solution, pI 7.0, 1 mg/mL in 10 mM NaOH, 0.45 μm filtered
68599 UV-VIS detectable IEF Marker solution, pI 7.2, 1 mg/mL in 10 mM NaOH, 0.45 μm filtered
93001 UV-VIS detectable IEF Marker solution, pI 7.5, 1 mg/mL in 10 mM NaOH, 0.45 μm filtered
91589 UV-VIS detectable IEF Marker solution, pI 3.7, 1 mg/mL in 10 mM NaOH, 0.45 μm filtered
01383 UV-VIS detectable IEF Marker solution, pI 3.9, 1 mg/mL in 10 mM NaOH, 0.45 μm filtered
07044 UV-VIS detectable IEF Marker solution, pI 4.2, 1 mg/mL in 10 mM NaOH, 0.45 μm filtered
15877 UV-VIS detectable IEF Marker solution, pI 5.3, 1 mg/mL in 10 mM NaOH, 0.45 μm filtered
67673 UV-VIS detectable IEF Marker solution, pI 6.2, 1 mg/mL in 10 mM NaOH, 0.45 μm filtered
90423 UV-VIS detectable IEF Marker solution, pI 6.6, 1 mg/mL in 10 mM NaOH, 0.45 μm filtered
19107 UV-VIS detectable IEF Marker solution, pI 8.4, 1 mg/mL in 10 mM NaOH, 0.45 μm filtered
75718 UV-VIS detectable IEF Marker solution, pI 9.0, 1 mg/mL in 10 mM NaOH, 0.45 μm filtered
52033 UV-VIS detectable IEF Marker solution, pI 9.6, 1 mg/mL in 10 mM NaOH, 0.45 μm filtered
39881 UV-VIS detectable IEF Marker solution, pI 10.1, 1 mg/mL in 10 mM NaOH, 0.45 μm filtered
36035 UV-VIS detectable IEF Marker solution, pI 10.4, 1 mg/mL in 10 mM NaOH, 0.45 μm filtered

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