|Related Categories||Alphabetical Index, Antibodies, Atto 488, Biochemicals and Reagents, Fluorescent Conjugates,|
|concentration||1 mg/mL protein|
|fluorescence||λex 485 nm; λem 552 nm in PBS|
|shipped in||wet ice|
Unconjugated dye ≤5% of total fluorescence; dye-to-protein ratio >2.
Atto 488 goat anti-rabbit IgG (whole molecule) is provided in unit sizes of 1 ml as 1 mg/ml solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.
Goat polyclonal anti-Rabbit IgG (whole molecule)-Atto 488 antibody may be used to detect and quantitate the level of IgG in rabbit serum and biological fluids via fluorogenic immunochemical or immunohistochemical techniques. It may also be used as a secondary antibody in assays that use rabbit IgG as the primary antibody. Atto 488 goat anti-rabbit IgG antibody was used for immunofluorescence to detect L. monocytogenes in mouse tissue sections following incubation with an anti-listeria antibody. Antibody was used at a 1:200 dilution. 1
Goat polyclonal anti-Rabbit IgG (whole molecule)-Atto 488 antibody antibody reats with rabbit IgG in vitro, in rabbit serum and biological fluids.
Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Atto 488 is a labeling dye with high molecular absorption (90,000) and quantum yield (0.80) as well as sufficient Stokes shift between excitation and emission maximum. It is optimized for excitation with an argon laser, and is characterized by high photostability. Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
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