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18846 Sigma

Phalloidin–Atto 655

suitable for fluorescence, ≥95.0% (sum of isomers, HPCE)

Synonym: Atto 655 Phalloidin

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Properties

Related Categories In Vivo/In Vitro Tracking of Stem Cells, Actin Inhibitors and Probes, Cell Biology, Cell Signaling and Neuroscience, Cytoskeleton and Extracellular Matrix,
assay   ≥95.0% (sum of isomers, HPCE)
fluorescence   λex 663 nm; λem 684 nm in 0.1 M phosphate pH 7.0
suitability   suitable for fluorescence
storage temp.   −20°C

Description

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Application

Fluorescent conjugates of phalloidin, rhodamine-phalloidin staining reagents, such as Phalloidin–Atto 655 are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.

General description

Atto 655 belongs to a new generation of fluorescent labels. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, good fluorescence quantum yield, excellent thermal and photo-stability, outstanding ozone resistance, very good water solubility, and very little triplet formation. Atto 655 is a zwitterionic dye with a net electrical charge of zero. The fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc.Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10–8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours.

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Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Superior Fluorescent Imaging Using Atto Dyes

Activated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescen...
BioFiles 2011, 6.3, 5.
Keywords: Absorption, Applications, Bacterial conjugations, Confocal microscopy, Degradations, Enzyme Assays, Extinction coefficient, Flow cytometry, In Situ hybridization, Infrared spectroscopy, Isomerizations, Microarray Analysis, Microscopy, Molecular probes, Nucleic acid hybridization, Protocols, Semiconductor, Separation, Spectroscopy, Support

Peer-Reviewed Papers
15

References

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