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24365C Sigma

EX-CELL® CD CHO Fusion

Animal-component free, without L-glutamine, without hypoxanthine, without sodium bicarbonate, without thymidine, dry powder, suitable for cell culture

Synonym: EX-CELL® CD CHO Serum-Free Medium for CHO Cells, Chemically Defined

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Properties

Related Categories CHO Media and Medium Optimization Kits, CHO Platform, Cell Culture, Serum-free Media
Quality Level   GMP
form   dry powder
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Application

EX-CELL ™ CD CHO FUSION is a chemically defined, animal-component free medium developed for the long-term growth of Chinese Hamster Ovary (CHO) cells. The absence of any large macromolecules allows for isolation and purification of secreted proteins from the cells.

This medium is supplied without L-glutamine to aid in media stability, to avoid L-glutamine degradation that causes ammonia build-up, and to provide an appropriate medium for the culture of CHO cells using the Glutamine Synthetase, or GS System™. This medium does not contain hypoxanthine or thymidine to allow for its use with dihydrofolate reductase (DHFR) gene amplification systems.

Quantity

Formulated to contain 20.1 grams of powder per liter of medium.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

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Protocols & Articles

Articles

CHOZN® Platform Technical Bulletin

Product Description/Overview Precautions and Disclaimer Storage and Stability Background Part I: Medium Preparation Part II: Stock culture initiation/thawing and subculturing Part III: Stock culture ...
Keywords: Amplification, Antibiotics, Buffers, Cell culture, Centrifugation, Cloning, Condensations, Cryopreservation, Degradations, Eliminations, Enzyme activity, Enzyme-linked immunosorbent assay, Filtration, Gene expression, Growth factors, High performance liquid chromatography, Metabolism, Metabolites, Pharmaceutical, Phase transitions, Precipitation, Purification, Titrations, Transfection

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