Synonym: Acid blue 90, Coomassie Brilliant Blue G
|Related Categories||B, Bioactive Small Molecules, Cell Biology, Molecular Biology, Protein Sequencing,|
λmax. ∼610 nm; E(1%,1 cm) ∼500-700 (H2O)
P2X7 purinergic receptor antagonist.
Protein stain for gel electrophoresis. Does not penetrate the gel and is three times more sensitive than Naphthol Blue Black B1; Simple and sensitive protein assay2; Rapid, sensitive and versatile assay for proteins; free amino acids and several chemicals that interfere with the Lowry procedure cause no interference3.
1. W.J. Shaphonov Meth. Enzymol. 11, 184, (1967)
Reconfiguring the quorum-sensing regulator SdiA of Escherichia coli to control biofilm formation via indole and N-acylhomoserine lactones. Jintae Lee et al Appl. Environ. Microbiol. 75, 1703-16, (2009)
A novel 4-(tetrahydro-2-furanmethoxy)-N-octadecyl-1,8-naphthalimide based blue emitiing probe: solvent effect on the photophysical properties and protein detection. Wei S, Sun Y, Guo P, et al. Bioorg. Khim. 38(5), 535-44, (2012)
Contrast recognizability during brilliant blue G - and heavier-than-water brilliant blue G-assisted chromovitrectomy: a quantitative analysis. Henrich PB, Valmaggia C, Lang C, et al. Acta Ophthalmol. 91(2), e120-4, (2013)
Phototoxicity of indocyanine green and Brilliant Blue G under continuous fluorescent illumination on cultured human retinal pigment epithelial cells. Takayama K, Sato T, Karasawa Y, et al. Invest. Ophthalmol. Vis. Sci. 53(11), 7389-94, (2012)
The unravelling of metabolic dysfunctions linked to metal-associated diseases by blue native polyacrylamide gel electrophoresis. Han S, Auger C, Castonguay Z, et al. Anal. Bioanal. Chem 405(6), 1821-31, (2013)
Microglia proliferation is controlled by P2X7 receptors in a Pannexin-1-independent manner during early embryonic spinal cord invasion. Rigato C, Swinnen N, Buckinx R, et al. J. Neurosci. 32(34), 11559-73, (2012)
Quantification of AAV particle titers by infrared fluorescence scanning of coomassie-stained sodium dodecyl sulfate-polyacrylamide gels. Kohlbrenner E, Henckaerts E, Rapti K, et al. Hum. Gene Ther. Methods 23(3), 198-203, (2012)
FasL-triggered death of Jurkat cells requires caspase 8-induced, ATP-dependent cross-talk between Fas and the purinergic receptor P2X(7). Aguirre A, Shoji KF, Sáez JC, et al. J. Cell Physiol. 228(2), 485-93, (2013)
Post-treatment change in the localization of recurrent or persistent macular edema secondary to branch retinal vein occlusion. Arima M, Miyazaki M, Arakawa S, et al. Ophthalmologica 228(1), 1-6, (2012)
Mechanism of Coomassie Brilliant Blue G-250 binding to cetyltrimethylammonium bromide: an interference with the Bradford assay. Aminian M, Nabatchian F, Vaisi-Raygani A, et al. Anal. Biochem. 434(2), 287-91, (2013)
High resolution quantitative proteomics of HeLa cells protein species using stable isotope labeling with amino acids in cell culture(SILAC), two-dimensional gel electrophoresis(2DE) and nano-liquid chromatograpohy coupled to an LTQ-OrbitrapMass spectrometer. Thiede B, Koehler CJ, Strozynski M, et al. Mol. Cell. Proteomics 12(2), 529-38, (2013)
Comparison of vitrectomy with brilliant blue G or indocyanine green on retinal microstructure and function of eyes with macular hole. Baba T, Hagiwara A, Sato E, et al. Ophthalmology 119(12), 2609-15, (2012)
In vitro secretion and activity profiles of matrix metalloproteinases, MMP-9 and MMP-2, in human term extra-placental membranes after exposure to Escherichia coli. Zaga-Clavellina V Reprod. Biol. Endocrinol. 9, 13, (2011)
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