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27815 Sigma

Brilliant Blue G

250, for microscopy

Synonym: Acid blue 90, Coomassie Brilliant Blue G

  • CAS Number 6104-58-1

  • Empirical Formula (Hill Notation) C47H48N3NaO7S2

  • Molecular Weight 854.02

  •  Beilstein Registry Number 5230822

  •  Colour Index Number 42655

  •  EC Number 228-058-4

  •  MDL number MFCD00078482

  •  PubChem Substance ID 24856509

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Description

Analysis Note

λmax. ∼610 nm; E(1%,1 cm) ∼500-700 (H2O)

Biochem/physiol Actions

P2X7 purinergic receptor antagonist.

Other Notes

Protein stain for gel electrophoresis. Does not penetrate the gel and is three times more sensitive than Naphthol Blue Black B4; Simple and sensitive protein assay5; Rapid, sensitive and versatile assay for proteins; free amino acids and several chemicals that interfere with the Lowry procedure cause no interference6.

Application

Brilliant Blue G was used to prepare the protein reagent for the determination of protein content of the collagenase enzyme isolated from fish waste.1 It may be employed as stain for the internal limiting membrane (ILM) for the macular hole (MH) and epiretinal membrane (ERM) surgery.2

General description

Brilliant Blue G (BBG) is a dye.2 It has been proposed as a substitute for internal limiting membrane (ILM) peeling.3

Price and Availability

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Poly(vinyl alcohol)

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Toluene
244511Toluene

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Safety & Documentation

Safety Information

WGK Germany 
3

Protocols & Articles

Peer-Reviewed Papers

References

Set your institution to view full text papers.

1. Isolation and activation of collagenase from fish processing waste. Daboor SM, et al. Adv. Biosci. Biotechnol. 3(3), (2012)

2. Brilliant blue G selectively stains the internal limiting membrane/brilliant blue G-assisted membrane peeling. Enaida H, Hisatomi T, Hata Y, et al. Retina (Philadelphia, Pa.) 26(6), 631-6, (2006)

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3. An in vivo evaluation of Brilliant Blue G in animals and humans. Remy M, Thaler S, Schumann RG, et al. Br. J. Ophthalmol. 92(8), 1142-7, (2008)

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4. W.J. Shaphonov Meth. Enzymol. 11, 184, (1967)

5. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye-binding. Bradford, M. Anal. Biochem. 72, 248-254, (1976)

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6. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250. Sedmak and Grossberg Anal. Biochem. 79, 544, (1977)

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Microglia proliferation is controlled by P2X7 receptors in a Pannexin-1-independent manner during early embryonic spinal cord invasion. Rigato C, Swinnen N, Buckinx R, et al. J. Neurosci. 32(34), 11559-73, (2012)

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High resolution quantitative proteomics of HeLa cells protein species using stable isotope labeling with amino acids in cell culture(SILAC), two-dimensional gel electrophoresis(2DE) and nano-liquid chromatograpohy coupled to an LTQ-OrbitrapMass spectrometer. Thiede B, Koehler CJ, Strozynski M, et al. Mol. Cell. Proteomics 12(2), 529-38, (2013)

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Cooperative binding of the outer arm-docking complex underlies the regular arrangement of outer arm dynein in the axoneme. Owa M, Furuta A, Usukura J, et al. Proc. Natl. Acad. Sci. U. S. A. 111(26), 9461-6, (2014)

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Epiretinal membrane recurrence: incidence, characteristics, evolution, and preventive and risk factors. Sandali O, El Sanharawi M, Basli E, et al. Retina (Philadelphia, Pa.) 33(10), 2032-8, (2013)

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Tyrosine phosphorylation of tau by the SRC family kinases lck and fyn. Timothy ME Scales et al Molecular Neurodegeneration 6, 12, (2011)

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Contrast recognizability during brilliant blue G - and heavier-than-water brilliant blue G-assisted chromovitrectomy: a quantitative analysis. Henrich PB, Valmaggia C, Lang C, et al. Acta Ophthalmol. 91(2), e120-4, (2013)

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[Protein determination by binding with the dye Coomassie brilliant blue G-250]. Gasparov VS and Degtiar' VG Biokhimiia 59(6), 763-77, (1994)

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Accidental subretinal brilliant blue G migration during internal limiting membrane peeling surgery. Almeida FP, De Lucca AC, Scott IU, et al. JAMA Ophthalmol. 133(1), 85-8, (2015)

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Brilliant blue G assisted macular surgery: the effect of air infusion on contrast recognisability in internal limiting membrane peeling. Totan Y, Güler E, Gürağaç FB, et al. Br. J. Ophthalmol. 99(1), 75-80, (2015)

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A novel 4-(tetrahydro-2-furanmethoxy)-N-octadecyl-1,8-naphthalimide based blue emitiing probe: solvent effect on the photophysical properties and protein detection. Wei S, Sun Y, Guo P, et al. Bioorg. Khim. 38(5), 535-44, (2012)

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I see colors: using vital dyes in diagnosing ophthalmic disease. McElhiney LF Int. J. Pharm. Compd. 16(3), 190-5, (2012)

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Sodium dodecyl sulphate-polyacrylamide denaturing gel electrophoresis and Western blotting techniques. Blancher C and Cormick RM Methods Mol. Biol. 878, 89-110, (2012)

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Fast and sensitive coomassie staining in quantitative proteomics. Dyballa N and Metzger S Methods Mol. Biol. 893, 47-59, (2012)

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Lipopolysaccharide inhibits the channel activity of the P2X7 receptor. Leiva-Salcedo E Mediators Inflamm. 2011, 152625, (2011)

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Dynamic increase in extracellular ATP accelerates photoreceptor cell apoptosis via ligation of P2RX7 in subretinal hemorrhage. Notomi S, Hisatomi T, Murakami Y, et al. PLoS ONE 8(1), e53338, (2013)

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Comparison of vitrectomy with brilliant blue G or indocyanine green on retinal microstructure and function of eyes with macular hole. Baba T, Hagiwara A, Sato E, et al. Ophthalmology 119(12), 2609-15, (2012)

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P2X7 blockade attenuates murine lupus nephritis by inhibiting activation of the NLRP3/ASC/caspase 1 pathway. Zhao J, Wang H, Dai C, et al. Arthritis Rheum. 65(12), 3176-85, (2013)

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A safe, blood-brain barrier permeable triphenylmethane dye inhibits amyloid-β neurotoxicity by generating nontoxic aggregates. Wong HE, Qi W, Choi HM, et al. ACS Chem. Neurosci. 2(11), 645-57, (2011)

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Clinical evaluation and feasibility of changing intraoperative visibility with a novel viewing filter system for human eye. Enaida H, Ikeda Y, Yoshida S, et al. Retina (Philadelphia, Pa.) 33(9), 1923-30, (2013)

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Reconfiguring the quorum-sensing regulator SdiA of Escherichia coli to control biofilm formation via indole and N-acylhomoserine lactones. Jintae Lee et al Appl. Environ. Microbiol. 75, 1703-16, (2009)

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Survival potential of wild type cellulose deficient Salmonella from the feed industry. Lene K Vestby et al BMC Vet. Res. 5, 43, (2009)

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P2X7 receptor antagonism inhibits p38 mitogen-activated protein kinase activation and ameliorates neuronal apoptosis after subarachnoid hemorrhage in rats. Chen S, Ma Q, Krafft PR, et al. Crit. Care Med. 41(12), e466-74, (2013)

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Anti-prion activity of Brilliant Blue G. Iwamaru Y, Takenouchi T, Murayama Y, et al. PLoS ONE 7(5), e37896, (2012)

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Characterization of hepatitis C RNA-containing particles from human liver by density and size. Nielsen SU, Bassendine MF, Martin C, et al. J. Gen. Virol. 89(Pt 10), 2507-17, (2008)

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Interpretation of protein quantitation using the Bradford assay: comparison with two calculation models. Ku HK, Lim HM, Oh KH, et al. Anal. Biochem. 434(1), 178-80, (2013)

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Staining internal limiting membrane with a mixture of BBG and sodium hyaluronate. Uemoto R, Nakasato-Sonn H, Meguro A, et al. Br. J. Ophthalmol. 97(6), 690-3, (2013)

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FasL-triggered death of Jurkat cells requires caspase 8-induced, ATP-dependent cross-talk between Fas and the purinergic receptor P2X(7). Aguirre A, Shoji KF, Sáez JC, et al. J. Cell Physiol. 228(2), 485-93, (2013)

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The structural effect of intravitreal Brilliant blue G and Indocyanine green in rats eyes. Ooi YL, Khang TF, Naidu M, et al. Eye (Lond.) 27(3), 425-31, (2013)

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Quantitative analysis of vitreous and plasma concentrations of brilliant blue g after use as a surgical adjuvant in chromovitrectomy. Enaida H, Kumano Y, Ueno A, et al. Retina (Philadelphia, Pa.) 33(10), 2170-4, (2013)

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Phototoxicity of indocyanine green and Brilliant Blue G under continuous fluorescent illumination on cultured human retinal pigment epithelial cells. Takayama K, Sato T, Karasawa Y, et al. Invest. Ophthalmol. Vis. Sci. 53(11), 7389-94, (2012)

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Staining of the internal limiting membrane with the use of heavy brilliant blue G. Pelayes DE, Kuhn F, Folgar AM, et al. Ophthalmic Res. 48 Suppl 1, 21-5, (2012)

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Quantification of AAV particle titers by infrared fluorescence scanning of coomassie-stained sodium dodecyl sulfate-polyacrylamide gels. Kohlbrenner E, Henckaerts E, Rapti K, et al. Hum. Gene Ther. Methods 23(3), 198-203, (2012)

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Silencing of the P2X(7) receptor enhances amyloid-β phagocytosis by microglia. Ni J, Wang P, Zhang J, et al. Biochem. Biophys. Res. Commun. 434(2), 363-9, (2013)

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Mechanism of Coomassie Brilliant Blue G-250 binding to cetyltrimethylammonium bromide: an interference with the Bradford assay. Aminian M, Nabatchian F, Vaisi-Raygani A, et al. Anal. Biochem. 434(2), 287-91, (2013)

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In vitro secretion and activity profiles of matrix metalloproteinases, MMP-9 and MMP-2, in human term extra-placental membranes after exposure to Escherichia coli. Zaga-Clavellina V Reprod. Biol. Endocrinol. 9, 13, (2011)

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The unravelling of metabolic dysfunctions linked to metal-associated diseases by blue native polyacrylamide gel electrophoresis. Han S, Auger C, Castonguay Z, et al. Anal. Bioanal. Chem 405(6), 1821-31, (2013)

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The Bs20x22 anti-CD20-CD22 bispecific antibody has more lymphomacidal activity than do the parent antibodies alone. Tuscano JM Cancer Immunol. Immunother. 60(6), 771-80, (2011)

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Interaction of nucleic acids with Coomassie Blue G-250 in the Bradford assay. Wenrich BR and Trumbo TA Anal. Biochem. 428(2), 93-5, (2012)

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Residual internal limiting membrane after epiretinal membrane peeling: results of the Pan-American Collaborative Retina Study Group. Carpentier C, Zanolli M, Wu L, et al. Retina (Philadelphia, Pa.) 33(10), 2026-31, (2013)

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Analysis of the Plasmodium falciparum proteasome using Blue Native PAGE and label-free quantitative mass spectrometry. Sessler N, Krug K, Nordheim A, et al. Amino Acids 43(3), 1119-29, (2012)

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Differences in purinergic amplification of osmotic cell lysis by the pore-forming RTX toxins Bordetella pertussis CyaA and Actinobacillus pleuropneumoniae ApxIA: the role of pore size. Masin J, Fiser R, Linhartova I, et al. Infect. Immun. 81(12), 4571-82, (2013)

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Photodynamic properties of vital dyes for vitreoretinal surgery. Brockmann T, Steger C, and Dawczynski J Ophthalmologica 228(4), 234-8, (2012)

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Post-treatment change in the localization of recurrent or persistent macular edema secondary to branch retinal vein occlusion. Arima M, Miyazaki M, Arakawa S, et al. Ophthalmologica 228(1), 1-6, (2012)

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Robust production of a peptide library using methodological synchronization. Huber KL Protein Expr. Purif. 67(2), 139-47, (2009)

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