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Atto 425-Biotin

BioReagent, suitable for fluorescence, ≥95.0% (HPCE)

Synonym: Biotin-Atto 425

  • Empirical Formula (Hill Notation) C37H53N5O7S

  • Molecular Weight 711.91

  •  MDL number MFCD04974818

  •  PubChem Substance ID 57648315

  •  eCl@ss 34058011

  • Popular Documents:  Datasheet (PDF)  

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Properties

Related Categories Biochemicals and Reagents, Biotin Conjugates, Fluorescent Conjugates, Fluorescent Probes, Labels, Particles and Stains
InChI Key   LPVYZZMUDCZDQO-UHFFFAOYSA-N
product line   BioReagent
assay   ≥95.0% (HPCE)
fluorescence   λex 437 nm; λem 483 nm in 0.1 M phosphate pH 7.0
suitability   suitable for fluorescence
storage temp.   −20°C

Description

General description

Atto 425 is a new label with high quantum yield (0.90) as well as sufficient stoke′s shift (excitation maximum 436 nm, emission maximum 484 nm). It is optimized for excitation with argon laser, and is characterized by high photostability. It does nearly not show cis-trans-isomerization, which limits brightness and reproducibility for many other dyes.

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 425 biotin is suitable for fluorescence labeling of avidin/streptavidin conjugated molecules.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Superior Fluorescent Imaging Using Atto Dyes

Activated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescen...
BioFiles 2011, 6.3, 5.
Keywords: Absorption, Applications, Bacterial conjugations, Confocal microscopy, Degradations, Enzyme Assays, Extinction coefficient, Flow cytometry, In Situ hybridization, Infrared spectroscopy, Isomerizations, Microarray Analysis, Microscopy, Molecular probes, Nucleic acid hybridization, Protocols, Semiconductor, Separation, Spectroscopy, Support

Peer-Reviewed Papers
15

References

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