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30396 Sigma

Cytochrome c from equine heart

≥90% (GE)

Synonym: Cytochrome c from horse heart

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Properties

Related Categories Cell Biology, Cytochrome c, Cytochromes, Electron Transport and Cellular Respiration, Metabolic Pathways,
assay   ≥90% (GE)
mol wt   Mr ~13000
impurities   ≤10% water
  ≤5% cytochrome c reduced
solubility   H2O: soluble10 mg/mL, clear, dark red-brown
storage temp.   −20°C

Description

Biochem/physiol Actions

Cytochrome c is primarily known as an electron-carrying mitochondrial protein. The transition of cytochrome c between the ferrous and ferric states within the cell makes it an efficient biological electron-transporter and it plays a vital role in cellular oxidations in both plants and animals. It is generally regarded as a universal catalyst of respiration, forming an essential electron-bridge between the respirable substrates and oxygen

Other Notes

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View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.

Preparation Note

By a procedure which completely avoids TCA and gives a highly native form.

Application

The specific sites and extent of oxidation in horse cytochrome c treated with H2O2 and UV were characterized. It was suggested that the state of these sites could be used as a biomarker for the oxidative environment in a cell.

Price and Availability

Safety & Documentation

Safety Information

WGK Germany 
3
RTECS 
HA5365000

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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