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30915 Sigma

Denhardt’s solution

BioReagent, for molecular biology, lyophilized powder

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Properties

grade   for molecular biology
product line   BioReagent
form   lyophilized powder
impurities   DNases, none detected
  RNases, none detected
  phosphatases, none detected
  proteases, none detected
suitability   suitable for nucleic acid hybridization
storage temp.   2-8°C

Description

Components

Each vial of Denhardt′s powder contains 50 mG Bovine Serum Albumin (BSA), 50 mG Ficoll, and 50 mG Polyvinylpyrrolidone (PVP).

Preparation Note

To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at −20°C for up to two years.

Application

Suitable to block non-specific binding sites in western, northern and Southern blot membranes

General description

Denhardt′s solution is a mixture of high-molecular weight polymers that act as blocking agents. It is used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
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