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32855 Sigma

Eosin Methylene blue

according to May-Grünwald

  •  Colour Index Number 45380/52015

  •  Colour Index Number 881/1038

  •  MDL number MFCD00005038

  •  PubChem Substance ID 329754361

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Properties

Related Categories Analytical Reagents, Analytical/Chromatography, Microscopy Reagents, Stains and Dyes
InChI Key   NXBZXYJXNIFCRJ-UHFFFAOYSA-M
quality   according to May-Grünwald

Description

General description

Mixture of eosin and methylene blue stains for use in differential microbiological media to culture and identify pathogenic microorganisms, e.g., Neisseria sicca,, Acinetobacter calcoaceticus.

Eosin has been used in combination with methylene blue, to detect carboxlic acid group in organic compounds. It gives a red or orange-coloured precipitate on treatment with carboxylic acid solution in water, acetone, alcohol, or dioxin. It is also used as an indicator for differentiation of the colon from the typhoid-dysentery group of bacteria.

Application

Eosin methylene blue was used for staining cells, during optical microscopy studies, done for analysis of cell morphology.

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Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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