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  • 35845 - 2′,7′-Dichlorofluorescein diacetate

35845 Sigma

2′,7′-Dichlorofluorescein diacetate

BioReagent, suitable for fluorescence, ≥95% (HPLC)

  • CAS Number 2044-85-1

  • Empirical Formula (Hill Notation) C24H14Cl2O7

  • Molecular Weight 485.27

  •  Beilstein Registry Number 68488

  •  MDL number MFCD00037501

  •  PubChem Substance ID 57649438



Related Categories Biochemicals and Reagents, Fluorescein and Derivatives, Fluorescent Labels, Fluorescent Probes, Labels, Particles and Stains, Luminescent Compounds/Detection,
product line   BioReagent
assay   ≥95% (HPLC)
mp   232-234 °C(lit.)
solubility   DMSO: soluble
fluorescence   λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)
suitability   suitable for fluorescence
storage temp.   2-8°C


Frequently Asked Questions

Frequently Asked Questions are available for this Product.


Do you have application information on this product that you would like to share?

2′,7′-Dichlorofluorescein diacetate may be used as a superior gas phase alternative to underivatized fluorescein and as a component/substrate of the 2′,7′-dichlorofluorescein diacetate assay to quantitate reactive oxygen species (ROS).

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Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What is required to get 2′,7′-Dichlorofluorescein diacetate, Product 35845, to fluoresce?
2',7'-dichlorofluorescein diacetate fluoresces only after cleavage of the acetyl groups.
In what solvents is 2′,7′-Dichlorofluorescein diacetate, Product 35845, soluble?
It is soluble in DMF, ethanol (2 mg/mL), DMSO (10 mg/mL).
What are the optical properties of the hydrolysis product of 2′,7′-Dichlorofluorescein diacetate, Product 35845?
Excitation maximum is at 504 nm; emission maximum is at 529 nm (in 0.1 M Tris pH 8.0 following treatment with an esterase).
What are the recommended storage conditions for 2′,7′-Dichlorofluorescein diacetate, Product 35845?
Product no. 35845 should be stored in a refrigerator or coldroom at 2-8°C.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Can Product 35845, 2′,7′-Dichlorofluorescein diacetate be used for ROS (Reactive Oxygen Species) detection?
Yes, Product 35845, 2′,7′-Dichlorofluorescein diacetate can be used for ROS detection by FACS analysis.  Click on the link to a reference below:  http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0069126  
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