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  • 35848 - 2′,7′-Dichlorofluorescein

35848 Sigma


suitable for fluorescence, BioReagent, ≥90% (T)

  • CAS Number 76-54-0

  • Empirical Formula (Hill Notation) C20H10Cl2O5

  • Molecular Weight 401.20

  •  Beilstein Registry Number 58009

  •  EC Number 200-968-6

  •  MDL number MFCD00005047

  •  PubChem Substance ID 57649439



Related Categories Alphabetical, Biochemicals and Reagents, Fluorescent Indicators and Probes, Fluorescent Probes, Labels, Particles and Stains, pH Sensitive Probes and Indicators More...
product line   BioReagent
assay   ≥90% (T)
mp   280 °C (dec.)(lit.)
fluorescence   λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0
suitability   suitable for fluorescence


Frequently Asked Questions

Frequently Asked Questions are available for this Product.


2′,7′-Dichlorofluorescein diacetate may be used as a superior gas phase alternative to underivatized fluorescein and as a component/substrate of the 2′,7′-dichlorofluorescein diacetate assay to quantitate reactive oxygen species (ROS).

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Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

What can be used to make a solution of 2′,7′-dichlorofluorescein?
2′,7′-Dichlorofluorescein is soluble at 25 mg/mL absolute ethanol with heating. 2′,7′-Dichlorofluorescein is also soluble in water at 0.1 mg/mL.
In what types of applications can 2′,7′-Dichlorofluorescein be used?
Some applications for use of 2′,7′-Dichlorofluorescein are listed below. This product can be used for the determination of carbohydrates by fluorescence densitometry after TLC.  See G. Gübitz et al., J. Chromatogr., 117, 337, (1976). 2′,7′-Dichlorofluorescein as also been used in the determination of H2O2 by chemiluminescence. See the following references by H. Watanabe et al. Anal. Sci. 2, 461, (1986) and T. Segawa et al. Anal. Sci. 6, 763, (1990). This product has also been used as a  fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. See W.D. Pfeffer and E.S. Yeung, J. Chromatogr., 506, 401, (1990).
What excitation /emission wavelengths are best for fluorescence detection of 2′,7′-Dichlorofluorescein?
2′,7′-Dichlorofluorescein can be detected at an excitation wavelength at 504 nm and emission wavelength at 529 nm in 0.1 M Tris pH 8.0.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
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Please view autoradiography film tips provided by Carestream Health, Inc. http://www.carestream.com/autoradiography-film-tips.html
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