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37294 Sigma

Dihydrofolate Reductase from bovine liver

~8 U/mL, ammonium sulfate suspension, off-white

Synonym: Tetrahydrofolate Dehydrogenase

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Properties

Related Categories 1.5.x.x Acting on CH-NH, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   ammonium sulfate suspension
activity   ≥6 U/mg protein
concentration   ~8 U/mL
color   off-white
density   1.2 g/mL at 20 °C
storage temp.   2-8°C

Description

Other Notes

In folate metabolism for the synthesis of purines, thymidine and glycine

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Unit Definition

1 U corresponds to the amount of enzyme which converts 1 μmol 7,8-dihydrofolate and NADPH to 5,6,7,8-tetrahydrofolate and NADP per minute at pH 6.5 and 25 °C

Physical form

Suspension in 3.6 M ammonium sulfate solution, pH 7.0

Application

Dihydrofolate reductase (DHFR) is involved in the synthesis of purines, thymidine and glycine in folate metabolism. DHFR, from bovine liver, is used for methotrexate analysis . DHFR is useful to study various cancers such as sarcomas, leukemias and head and neck cancers .

Biochem/physiol Actions

Dihydrofolate reductase (DHFR) reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as an electron donor. The binding of antitumor agents, such as methotrexate, to DHFR prevents the formation of reduced folates, which are essential for DNA synthesis .

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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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