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391A Sigma

Peroxidase (Myeloperoxidase) Leukocyte Kit

DAB substrate kit

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Properties

Related Categories Cell Biology, Hematology and Histology, Histology Special Stains More...
Quality Level   GMP-IVD
usage   sufficient for 10-120 manual assays
shelf life   Expiry date on the label.
IVD   for in vitro diagnostic use
shipped in   wet ice
storage temp.   2-8°C

Description

Application

Staining system for polymorphonucleocytes in blood or bone marrow films. The staining characteristics of polymorphonucleocytes are used to distinguish acute myelocytic leukemia from other types of leukemia.

Diaminobenzidine, as a benzidine substitute for myeloperoxidase, has less tinctorial power than benzidine. This made DAB much less attractive to histochemists. Hanker and associates improved DAB methodology. According to their modification, the brown reaction product is first intensified with copper salts and followed by application of Gill′s modified Papanicolaou stain, resulting in intense grey-black staining at sites of neutrophil and monocyte peroxidase.

General description

Used to demonstrate polymorphonuclear cells in blood or bone marrow films. Each kit has sufficient reagents for 10 uses.

Price and Availability


All labs need water

Kit component only

Description

Product #

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Diaminobenzidine 10 vials SDS    
Copper nitrate 2 vials SDS    
Gill Modified EA solution 100 mL SDS    
Glutaraldehyde solution 2 x 75 mL SDS    
Hematoxylin Solution, Gill No. 3 100 mL SDS    
Scott's Tap Water Substitute Concentrate 100 mL SDS    
TRIZMAL™ Buffer Concentrate 50 mL SDS    
Safety & Documentation

Safety Information

Signal word 
Danger
Precautionary statements 
RIDADR 
UN 3316 9
Flash Point(F) 
57.2 °F
Flash Point(C) 
14 °C

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
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S5134 Scott′s Tap Water Substitute Concentrate, 10 ×
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S4651 Silane-Prep Slides, glass slides coated with silane (aminoalkylsilane).
GHS332 Hematoxylin Solution, Gill No. 3
P8920 Poly-L-lysine solution, 0.1 % (w/v) in H2O
08168 Timestrip Plus 8 °C
3802 Glutaraldehyde solution, for in vitro diagnostic use

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