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395B Sigma

Periodic Acid-Schiff (PAS) Kit

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Properties

Related Categories Cell Biology, Hematology and Histology, Histology Special Stains More...
Quality Level   GMP-IVD
shelf life   Expiry date on the label.
IVD   for in vitro diagnostic use
storage temp.   2-8°C

Description

Application

Standard procedure and microwave procedure included in package insert.

When treated with periodic acid, glycols are oxidized to aldehydes. After reaction with Schiff′s Reagent (a mixture of pararosaniline and sodium metabisulfite), a pararosaniline adduct is released that stains the glycol-containing cellular elements pink to red. Cellular elements which may be demonstrated with the PAS procedure include glycogen, fungal walls, basement membrane, certain epithelial sulfomucins and sialomucins, neutral mucosubstances, colloid material of the thyroid and the pars intermedia of the pituitary.

Diagnostic Implications

Staining patterns of lymphocytes are helpful in making therapeutic decisions in established cases of lymphocytic leukemia.

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Standard component

Description

Product #

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Hematoxylin Solution, Gill No. 3 (kit only) 100 mL MSDS    
Periodic Acid Solution (395-1) 2 x 100 mL MSDS    
Schiff's Reagent (kit only) 4 x 50 mL MSDS    

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Periodic acid solution

1 g/dL in deionized water

Periodic acid solution

1 g/dL in deionized water

suitable for detection of glycoproteins

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Hazard Codes (Europe) 
Xn
Risk Statements (Europe) 
Safety Statements (Europe) 
26-39
RIDADR 
UN 3316 9
WGK Germany 
3

Protocols & Articles

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R8382 Reagent Alcohol, used for histology tissue preparation
395132 Periodic acid solution, 1 g/dL in deionized water
3951 Periodic acid solution, 1 g/dL in deionized water

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