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41969 Sigma

5-Carboxy-X-rhodamine N-succinimidyl ester

BioReagent, suitable for fluorescence

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Properties

Related Categories Biochemicals and Reagents, Detection, Fluorescent Labels, Fluorescent Probes, Labels, Particles and Stains, Fluorescent Stains,
InChI Key   BTTBJYLMDASSAS-UHFFFAOYSA-N
product line   BioReagent
solubility   DMF: soluble
  acetonitrile: soluble
fluorescence   λex 575 nm; λem 600 nm in 0.1 M phosphate pH 7.0
suitability   suitable for fluorescence
storage temp.   −20°C

Description

Other Notes

Labeling reagent for prepn. of charge-modified dye-labeled ddNTPs to "direct-load" DNA sequencing

Application

5-Carboxy-X-rhodamine N-succinimidyl ester (ROX-SE) is used as an amine coupling reagent to form 5-carboxy-X-rhodamine (ROX) derivatized compounds such as proteins, nucleic acids and drugs that may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications.Labeling reagent for preparation of charge-modified dye-labeled ddNTPs to "direct-load" DNA sequencing.

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Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Fluorescent Labeling of Peptides

Labeling peptides with fluorescent dyes or other labels provides powerful tools for the investigation of biological relevant interactions like receptor-ligand-binding,1-3 protein structures,4-6 and e...
ChemFiles Volume 5 Article 12
Keywords: Alzheimer Disease, Biochemistry, Electrophoresis, Enzyme activity, Immobilization, Ligands, Methods, Microscopy, Peptide synthesis, Separation, Solid phase peptide synthesis, Solvents, Spectroscopy, Tools

Peer-Reviewed Papers
15

References

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