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43208 Sigma

Atto 590-Biotin

BioReagent, suitable for fluorescence, ≥90.0% (HPCE)

Synonym: Biotin-Atto 590

  • Empirical Formula (Hill Notation) C52H65ClN6O10S

  • Molecular Weight 1001.62

  •  MDL number MFCD04040677

  • Popular Documents:  Datasheet (PDF)  

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Properties

Related Categories Biochemicals and Reagents, Biotin Conjugates, Fluorescent Conjugates, Fluorescent Probes, Labels, Particles and Stains
product line   BioReagent
assay   ≥90.0% (HPCE)
fluorescence   λex 590 nm; λem 625 nm in 0.1 M phosphate pH 7.0
suitability   suitable for fluorescence
storage temp.   −20°C

Description

General description

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Application

Atto 590 is a new label with high molecular absorption (120.000) and quantum yield (0.80) as well as sufficient stoke′s shift (excitation maximum 594 nm, emission maximum 624 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Biotin conjugates can be used in applications like ELISA or immunohistochemistry, in situ hybridization, flow cytometry and others, to identify streptavidin, avidin, or extravidin-conjugates.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Atto Dyes for Superior Fluorescent Imaging

Activated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescen...
BioFiles 2011, 6.3, 5.
Keywords: Absorption, Applications, Bacterial conjugations, Confocal microscopy, Degradations, Enzyme Assays, Extinction coefficient, Flow cytometry, In Situ hybridization, Infrared spectroscopy, Isomerizations, Microarray Analysis, Microscopy, Molecular probes, Nucleic acid hybridization, Protocols, Semiconductor, Separation, Spectroscopy, Support

Peer-Reviewed Papers
15

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