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43815 Sigma

DL-Dithiothreitol

BioUltra, for molecular biology, ≥99.5% (RT)

Synonym: threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT

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Properties

Related Categories Alphabetical, BioUltra, Biochemicals and Reagents, Carbohydrates, Denaturation,
grade   for molecular biology
InChI Key   VHJLVAABSRFDPM-QWWZWVQMSA-N
product line   BioUltra
assay   ≥99.5% (RT)
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
  ≤0.3% 4,5-dihydroxy-1,2-dithiane
pH   4.0-6.5 (25 °C, 0.1 M in H2O)
mp   41-44 °C(lit.)
solubility   H2O: soluble0.1 M at 20 °C, clear, colorless
anion traces   sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.5 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤500 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   0.1 M in H2O
UV absorption   λ: 260 nm Amax: 0.400
  λ: 280 nm Amax: 0.100
storage temp.   2-8°C

Description

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Other Notes

Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
UN 3335
WGK Germany 
3
RTECS 
EK1610000
Flash Point(F) 
235.4 °F
Flash Point(C) 
113 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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