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43817 Sigma

DL-Dithiothreitol

BioUltra, ≥99.0% (RT)

Synonym: threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT

  • CAS Number 3483-12-3

  • Linear Formula HSCH2CH(OH)CH(OH)CH2SH

  • Molecular Weight 154.25

  •  Beilstein Registry Number 1719757

  •  EC Number 222-468-7

  •  MDL number MFCD00004877

  •  PubChem Substance ID 57650197

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Properties

Related Categories Alphabetical, BioUltra, Biochemicals and Reagents, Carbohydrates, Denaturation,
InChI Key   VHJLVAABSRFDPM-QWWZWVQMSA-N
product line   BioUltra
assay   ≥99.0% (RT)
impurities   insoluble matter, passes filter test
  ≤0.3% 4,5-dihydroxy-1,2-dithiane
pH   4.0-6.5 (25 °C, 0.1 M in H2O)
mp   41-44 °C(lit.)
solubility   H2O: soluble0.1 M at 20 °C, clear, colorless
anion traces   sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.5 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤100 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   0.1 M in H2O
UV absorption   λ: 260 nm Amax: 0.400
  λ: 280 nm Amax: 0.100
storage temp.   2-8°C

Description

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Other Notes

Cleavage of disulfide bonds in proteins; In the investigation of incremental and kinetic reduction in proteins; Prevents the oxidation of cholecystokinin during its extraction from brain and duodenal tissues; In protein sequence analysis.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
UN 3335
WGK Germany 
3
RTECS 
EK1610000
Flash Point(F) 
235.4 °F
Flash Point(C) 
113 °C

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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