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43819 Sigma

DL-Dithiothreitol

≥99.0% (RT)

Synonym: threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT

  • CAS Number 3483-12-3

  • Linear Formula HSCH2CH(OH)CH(OH)CH2SH

  • Molecular Weight 154.25

  •  Beilstein Registry Number 1719757

  •  EC Number 222-468-7

  •  MDL number MFCD00004877

  •  PubChem Substance ID 329756409

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Properties

Related Categories Biochemicals and Reagents, Carbohydrates, Carbohydrates A to Z, Carbohydrates D-F, Chemical Synthesis,
InChI Key   VHJLVAABSRFDPM-QWWZWVQMSA-N
assay   ≥99.0% (RT)
impurities   ≤0.3% 4,5-dihydroxy-1,2-dithiane
mp   41-44 °C(lit.)
  42-43 °C
solubility   H2O: 50 mg/mL clear, colorless
  H2O: freely soluble
  acetone: freely soluble
  chloroform: freely soluble
  diethyl ether: freely soluble
  ethanol: freely soluble
  ethyl acetate: freely soluble
anion traces   sulfate (SO42-): ≤50 mg/kg
cation traces   Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Na: ≤250 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Zn: ≤5 mg/kg
storage temp.   2-8°C

Description

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

DL-Dithiothreitol is a pivotal component used in the preparation of buffer which in turn is utilized in the platelet cytosol preparation. It is also important in isolation of RNA by using guanidinium salts and homogenisation of tissue and cells.

Biochem/physiol Actions

DL-Dithiothreitol denatures proteins by reducing the disulphide bonds and reducing them to SH groups. It also prevents intramolecular and intermolecular disulfide bond formation between cysteine residues of proteins.

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Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
UN 3335
WGK Germany 
3
RTECS 
EK1610000
Flash Point(F) 
235.4 °F
Flash Point(C) 
113 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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