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44581 Sigma

DPX Mountant for histology

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

DPX Mountant for histology is a mixture of distyrene, a plasticizer, and xylene used as a synthetic resin mounting media, that replaces xylene-balsam. DPX Mountant dries quickly and preserves stain. DPX Mountant is suitable for HE- (Hematoxylin-Eosin) and Masson-Goldner staining.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Precautionary statements 
RIDADR 
UN 1993C 3 / PGIII
WGK Germany 
3
Flash Point(F) 
82.4 °F
Flash Point(C) 
28 °C

Documents

Certificate of Analysis

Frequently Asked Questions

How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/General_Information/shippingandlong-termstoragev3.Par.0001.File.tmp/shippingandlong-termstoragev3.pdf
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the classification of DPX Mountant for histology (Product No. 44581)?
DPX Mountant for histology is regarded as a permanent or a resinous mounting media.
Is Product No. 44581, DPX Mountant, soluble in water?
No, this mounting media is not soluble in water.
What is the solvent used to dissolve the resin of DPX Mountant, Product No. 44581?
Xylene is the solvent of choice for dissolving DPX Mountant.
Does DPX Mountant, Product No. 44581, dry rapidly or slowly?
DPX dries rapidly in comparison to either Canada balsam or gum dammar.
Typical histological protocols typically call for dehydrating, clearing and mounting in a permanent mounting media. How is this performed with DPX Mountant, Product No. 44581?
Dehydration refers to removing the water by using a series of grades of alcohols.  Typically slides spend 1-5 minutes each in a series of alcohols. Sequences vary by laboratory. A typical sequence would be 1 station of 80% alcohol, 2-3 changes of 95% alcohol, 2-3 changes of absolute alcohol. The slides are then processed through 2-3 changes of xylene. After finishing with the last xylene solution, the slides are ready for coverslipping with the DPX. In some special stain procedures, the time in the dehydration alcohols is shortened to reduce the possibility of the dye leaching from the stained slides during dehydration.  Typically 10-15 rapid dips in each alcohol is suitable. The purpose of dehydration is to remove water from the tissue sections. If the coverslipped slides are clear, the water was removed. If the coverslipped slides appear cloudy, some water was retained.
What are the specifications for the refractive index of DPX Mountant, Product No. 44581?
The product specifications allow for a refractive index between 1.518 to 1.520.
Why is refractive index important for mounting medias like DPX Mountant, Product No. 44581?
Tissue typically has an average refractive index of 1.53 or 1.54.  Most permanent mounting medias will have a refractive index ranging from 1.51 to 1.55.  Water by comparison has a refractive index of only 1.33.  The closer the refractive index of the tissue and the mounting media, the greater the clarity and resolution of the slide when viewed under a microscope.
Can the coverslip be removed once a slide has hardened when using DPX Mountant, Product No. 44581?
Yes. It is possible to remove the coverslip once the DPX has set. Typically the slide is immersed in Xylene. The coverslip may loosen in several minutes, several hours or after 24 hours or greater. Differences may occur due to the amount of mounting media originally applied at the time of coverslipping or the length of time the DPX was allowed to harden. At times it might be necessary to gently pry the edges of the coverslip. This may shorten the period needed for the coverslip to detach. The slide is then agitated in 3 or 4 changes of xylene.  The slides are then processed through 2-3 changes of absolute alcohol, 2-3 changes of 95% alcohol. The slides are then rinsed well in running tap water. The slides are now suitable for destaining and restaining.
My protocol calls for using Permount.  Can I substitute DPX Mountant (Product No. 44581) for histology?
Yes. Both products are considered permanent mounting medias. They are used for similar purposes.
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Protocols & Articles
Peer-Reviewed Papers
15

References

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06522 DPX Mountant for histology, slide mounting medium

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