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44583 Sigma

Doxorubicin hydrochloride

suitable for fluorescence, 98.0-102.0% (HPLC)

Synonym: DOX, Hydroxydaunorubicin hydrochloride

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Properties

Related Categories Antibiotics, Antibiotics A to Z, Antibiotics A-F, Antibiotics by Application, Antineoplastic and Immunosuppressive Antibiotics,
biological source   from synthetic
InChI Key   MWWSFMDVAYGXBV-RUELKSSGSA-N
assay   98.0-102.0% (HPLC)
form   solid
color   orange to dark red
mp   216 °C (dec.)(lit.)
solubility   H2O: 50 mg/mL clear, orange to red
  DMSO: soluble
  THF: soluble
  ethanol: soluble
  methanol: soluble
fluorescence   λex 470 nm; λem 585 nm in ethanol
suitability   suitable for fluorescence
Mode of action   DNA synthesis | interferes
  enzyme | inhibits
antibiotic activity spectrum   neoplastics
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Do you have application information on this product that you would like to share?

Doxorubicin was used to induce apoptosis, and to induce cardiotoxicity in SK-N-MC cells . It is used to inhibit macromolecular biosynthesis.

Packaging

1, 10, 50 mg in glass bottle

Bottomless glass bottle. Contents are inside inserted fused cone.

Biochem/physiol Actions

Doxorubicin ia an antitumor antibiotic that intercalates DNA and inhibits macromolecular biosynthesis. DNA replication is haulted due to the inhibition of topoisomerase II. It is an inhibitor of reverse transcriptase and RNA polymerase and is an immunosuppressive agent .

Other Notes

Keep container tightly closed in a dry and well-ventilated place.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
QI9295900

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
What is the function of Doxorubicin hydrochloride, Product 44583?
In chemotherapy: "Doxorubicin HCl is the hydrochloride salt of doxorubicin, an anthracycline antibiotic with antineoplastic activity. Doxorubicin, isolated from the bacterium Streptomyces peucetius var. caesius, is the hydroxylated congener of daunorubicin."  -National Cancer Institute.
At what concentration can Doxorubicin hydrochloride, Product 44583, be dissolved in water? At what concentration in DMSO?
The solubility of Product No. 44583 tested in water (50 mg/mL), yielding a clear orange to red solution.  It is also soluble in DMSO (50 mg/mL), ethanol, methanol and tetrahydrofuran.
How stable is a solution of Doxorubicin hydrochloride, Product 44583?
According to the 11th edition of the Merck Index, "aqueous solutions are unchanged after one month at 5 °C but unstable at higher temperatures or at either acid or alkaline pH.  Several studies cite that stability is related to pH, exposure to light and the medium used for storage. Doxorubicin hydrochloride is subject to light degradation at <0.5 mg/mL, with considerable loss of bioactivity. If stored at 25 °C in the dark in 5% glucose, pH 4.7 (or 3.3% glucose + 0.3% sodium chloride, pH 4.4), solutions were stable (5% or less change) for at least 4 weeks."
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
How should I use Product D1515 - Doxorubicin hydrochloride to induce double strand breaks in the DNA of HeLa cells?
We do not test Product D1515 - Doxorubicin hydrochloride for biological activity with HELA cells.   The following paper used 100 nM to 250 nM doxorubicin   Adam Hermawan, et al.,  Journal of Natural Remedies, Vol. 12/2 (2012) 108-114 The following paper uses  0.01 to 1.0 mug/ml doxorubicin   SH Kim, et al., CANCER RESEARCH 32, 323-325, February 1972
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Protocols & Articles

Articles

Applications of Graphene Oxide and Reduced Graphene Oxide

Graphene oxide (GO, Aldrich product numbers 763705 and 777676) is a unique material that can be viewed as a single monomolecular layer of graphite with various oxygen containing functionalities such ...
Avery Luedtke, Ph.D.
Aldrich Materials Science, Sigma-Aldrich Co. LLC
Keywords: Cancer, Catalysis, Materials Science, Nanomaterials, Reductions, Semiconductor, Solar cells

Inhibition of Nucleic Acid Synthesis by Antibiotics

Quinolones are a key group of antibiotics that interfere with DNA synthesis by inhibiting topoisomerase, most frequently topoisomerase II (DNA gyrase), an enzyme involved in DNA replication. DNA gyra...
BioFiles 2006, 1.4, 7.
Keywords: Antibiotics, Antitumor agents, Cancer, DNA replication

Peer-Reviewed Papers
15

References

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