• USA Home
  • 45124 - Elastase from porcine pancreas

EMAIL THIS PAGE TO A FRIEND
45124 Sigma

Elastase from porcine pancreas

powder, white, ≥15 U/mg

Synonym: Elastase from hog pancreas, Pancreatopeptidase E

Purchase

Properties

Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
form   powder
mol wt   Mr ~25000
color   white
storage temp.   −20°C

Description

Biochem/physiol Actions

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Other Notes

Cleaves polyalanyl-lysine peptides at the alanyl-lysine bond, review; Review, elastase-type proteases

Sales restrictions may apply

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol 4-nitroaniline per minute at 25°C and pH 7.8 (succinyl-(L-Ala)3-4-nitroanilide as substrate)

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

Price and Availability


Laboratory Gloves

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

HPLC Analysis of Elastase on Discovery® BIO PolyMA-SCX, Ion Exchange

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Elastase on Discovery® BIO PolyMA-SCX, Ion Exchange
Keywords: Chromatography, High performance liquid chromatography

Protocols

Elastase (EC 3.4.21.36) continuous spectrophotometric rate assay

The continuous spectrophotometric rate determination (A410, Light path = 1 cm) is based on the following reaction:
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?