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45124 Sigma

Elastase from porcine pancreas

powder, white, ≥15 U/mg

Synonym: Elastase from hog pancreas, Pancreatopeptidase E

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Properties

Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
form   powder
mol wt   Mr ~25000
color   white
storage temp.   −20°C

Description

Biochem/physiol Actions

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Other Notes

Cleaves polyalanyl-lysine peptides at the alanyl-lysine bond, review; Review, elastase-type proteases

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Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol 4-nitroaniline per minute at 25°C and pH 7.8 (succinyl-(L-Ala)3-4-nitroanilide as substrate)

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Elastase (EC 3.4.21.36) continuous spectrophotometric rate assay

The continuous spectrophotometric rate determination (A410, Light path = 1 cm) is based on the following reaction:
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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