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45125 Sigma

Elastase from porcine pancreas

lyophilized, powder, white, ~8 U/mg

Synonym: Elastase from hog pancreas, Pancreatopeptidase E

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Properties

Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
form   powder
quality   lyophilized
mol wt   Mr ~25000
impurities   salt, none detected
color   white
storage temp.   −20°C

Description

Biochem/physiol Actions

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Other Notes

For protein sequence studies, limited proteolysis; Application in (selective) hydrolysis/condensation of carboxylic ester bonds

Sales restrictions may apply

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol 4-nitroaniline per minute at 25°C and pH 7.8 (succinyl-(L-Ala)3-4-nitroanilide as substrate)

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

Price and Availability


Laboratory Gloves

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

HPLC Analysis of Elastase on Discovery® BIO PolyMA-SCX, Ion Exchange

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Elastase on Discovery® BIO PolyMA-SCX, Ion Exchange
Keywords: Chromatography, High performance liquid chromatography

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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