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46043 Sigma

Ethidium homodimer

suitable for fluorescence, ~90% (HPCE)

Synonym: EtDi, EthD-1

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Properties

Related Categories Alphabetical, Biochemicals and Reagents, Detection, Fluorescent Enzyme Substrates, Fluorescent Enzyme Substrates for Electrophoresis,
InChI Key   ZKAJUKABUUJRLB-UHFFFAOYSA-N
assay   ~90% (HPCE)
mp   ≥250 °C(lit.)
solubility   DMF: soluble
  DMSO: soluble
  H2O: soluble
fluorescence   λex 493 nm in H2O
  λex 528 nm; λem 617 nm in TE buffer; DNA
suitability   suitable for fluorescence
storage temp.   −20°C

Description

Application

Staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. It will not cross intact cell membranes and can be used to test cell viability.

Ethidium homodimer (EtDi, EthD-1) is used as a staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. EthD-1 does not cross intact/viable cell membranes and can be used to test cell viability.

Other Notes

Reagent for the fluorimetric detection of nucleic acids

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Personal Protective Equipment 
RIDADR 
UN 3096 4.3(8) / PGII
WGK Germany 
3
Protocols & Articles

Articles

Nancy-520 for DNA Detection and Quantitation

Nancy-520 is a fluorescent stain for double stranded DNA (dsDNA) on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is...
Monika Baeumle
BioFiles 2009, 4.1, 4.
Keywords: Catalytic combustion detector, Electrophoresis

Peer-Reviewed Papers
15

References

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