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47614 Sigma

Fluram

BioReagent, suitable for fluorescence, ≥99.0% (UV)

Synonym: 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione, Fluorescamine

  • CAS Number 38183-12-9

  • Empirical Formula (Hill Notation) C17H10O4

  • Molecular Weight 278.26

  •  Beilstein Registry Number 921143

  •  EC Number 253-814-5

  •  MDL number MFCD00005928

  •  PubChem Substance ID 329757329

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Properties

Related Categories Alphabetical, Amino Acid Analysis, Amino Acids, Analytical Reagents, Analytical/Chromatography,
InChI Key   ZFKJVJIDPQDDFY-UHFFFAOYSA-N
product line   BioReagent
assay   ≥99.0% (UV)
mp   153-157 °C(lit.)
  153-157 °C
solubility   acetonitrile: soluble
  ethanol: soluble
fluorescence   λex 234 nm
  λex 390 nm; λem 480 nm in 0.5 M borate pH 8.5 (after derivatization with L-leucine)
suitability   suitable for fluorescence

Description

Application

Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.

Fluram (fluorescamine) is a non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides and other molecules to form stable, highly fluorescent compounds. Fluorescamine is useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes and as a pre-column derivatization reagent. It effectively blocks newly generated amino termini in protein sequence analyses.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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