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47671 Sigma

Formamide

BioUltra, for molecular biology, ≥99.5% (T)

Synonym: Amide C1, Formic amide

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Properties

Related Categories Acids and Bases, Alphabetical, BioUltra, Biochemicals and Reagents, DNA & RNA Purification,
grade   for molecular biology
vapor density   1.55 (vs air)
vapor pressure   0.08 mmHg ( 20 °C)
  30 mmHg ( 129 °C)
InChI Key   ZHNUHDYFZUAESO-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.5% (T)
autoignition temp.   932 °F
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
ign. residue   ≤0.1%
refractive index   n20/D 1.447(lit.)
  n20/D 1.447
pH   7-9 (25 °C, 1 M in H2O)
bp   210 °C(lit.)
mp   2-3 °C(lit.)
solubility   H2O: soluble10 M at 20 °C, clear, colorless
density   1.134 g/mL at 25 °C(lit.)
anion traces   chloride (Cl-): ≤1 mg/kg
  sulfate (SO42-): ≤10 mg/kg
cation traces   Ag: ≤5 mg/kg
  Al: ≤1 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤1 mg/kg
  Bi: ≤1 mg/kg
  Ca: ≤5 mg/kg
  Cd: ≤1 mg/kg
  Co: ≤1 mg/kg
  Cr: ≤1 mg/kg
  Cu: ≤1 mg/kg
  Fe: ≤1 mg/kg
  K: ≤20 mg/kg
  Li: ≤1 mg/kg
  Mg: ≤1 mg/kg
  Mn: ≤1 mg/kg
  Mo: ≤1 mg/kg
  NH4+: ≤1000 mg/kg
  Na: ≤20 mg/kg
  Ni: ≤1 mg/kg
  Pb: ≤1 mg/kg
  Sr: ≤1 mg/kg
  Tl: ≤5 mg/kg
  Zn: ≤1 mg/kg
λ   10 M in H2O
UV absorption   λ: 260 nm Amax: ≤0.4
  λ: 280 nm Amax: ≤0.1

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Other Notes

For the preparation of formamide denaturing polyacrylamide gels; In situ hybridization on human chromosomes

Price and Availability


Microcon Centrifugal Filters

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Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Target organs 
Blood
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
LQ0525000
Flash Point(F) 
347 °F
Flash Point(C) 
175 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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