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47671 Sigma

Formamide

BioUltra, for molecular biology, ≥99.5% (T)

Synonym: Amide C1, Formic amide

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Properties

Related Categories Acids and Bases, Alphabetical, BioUltra, Biochemicals and Reagents, DNA & RNA Purification,
grade   for molecular biology
vapor density   1.55 (vs air)
vapor pressure   0.08 mmHg ( 20 °C)
  30 mmHg ( 129 °C)
InChI Key   ZHNUHDYFZUAESO-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.5% (T)
autoignition temp.   932 °F
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
ign. residue   ≤0.1%
refractive index   n20/D 1.447(lit.)
  n20/D 1.447
pH   7-9 (25 °C, 1 M in H2O)
bp   210 °C(lit.)
mp   2-3 °C(lit.)
solubility   H2O: 10 M at 20 °C clear, colorless
density   1.134 g/mL at 25 °C(lit.)
anion traces   chloride (Cl-): ≤1 mg/kg
  sulfate (SO42-): ≤10 mg/kg
cation traces   Ag: ≤5 mg/kg
  Al: ≤1 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤1 mg/kg
  Bi: ≤1 mg/kg
  Ca: ≤5 mg/kg
  Cd: ≤1 mg/kg
  Co: ≤1 mg/kg
  Cr: ≤1 mg/kg
  Cu: ≤1 mg/kg
  Fe: ≤1 mg/kg
  K: ≤20 mg/kg
  Li: ≤1 mg/kg
  Mg: ≤1 mg/kg
  Mn: ≤1 mg/kg
  Mo: ≤1 mg/kg
  NH4+: ≤1000 mg/kg
  Na: ≤20 mg/kg
  Ni: ≤1 mg/kg
  Pb: ≤1 mg/kg
  Sr: ≤1 mg/kg
  Tl: ≤5 mg/kg
  Zn: ≤1 mg/kg
λ   10 M in H2O
UV absorption   λ: 260 nm Amax: ≤0.4
  λ: 280 nm Amax: ≤0.1

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Other Notes

For the preparation of formamide denaturing polyacrylamide gels; In situ hybridization on human chromosomes

Price and Availability


Microcon Centrifugal Filters

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Target organs 
Blood
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
LQ0525000
Flash Point(F) 
347 °F
Flash Point(C) 
175 °C

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
What is the best way to deionize Formamide, Product 47671?
Mixed Bed Resin (e.g., Sigma Product No. M8157 or M8032) can be used to deionize formamide.
Are aqueous solutions of Formamide, Product 47671, stable?
In aqueous solution, formamide hydrolyzes to form ammonia and formic acid (i.e., ammonium formate).
What is the molarity of the neat liquid Formamide, Product 47671?
Based on the density of 1.13 and the molecular weight of 45.04, the molarity of this neat liquid is 25.09 M. The pH of a 0.5 M aqueous solution is 7.1, according to The Merck Index, 12th edition.
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

GC Analysis of Class 2 Residual Solvents on OVI-G43

From our library of Articles, Sigma-Aldrich presents GC Analysis of Class 2 Residual Solvents on OVI-G43
Keywords: Chromatography, Flame ionization detector, Gas chromatography, Pharmaceutical, Size-exclusion chromatography

Peer-Reviewed Papers
15

References

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