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49767 Sigma

Glycerol

BioUltra, for molecular biology, anhydrous, ≥99.5% (GC)

Synonym: 1,2,3-Propanetriol, Glycerin

  • CAS Number 56-81-5

  • Linear Formula HOCH2CH(OH)CH2OH

  • Molecular Weight 92.09

  •  Beilstein Registry Number 635685

  •  EC Number 200-289-5

  •  MDL number MFCD00004722

  •  PubChem Substance ID 24873136

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Properties

Related Categories Alphabetical, BioUltra, Biochemicals and Reagents, Biostabilization Agents, Cell Biology,
grade   for molecular biology
vapor density   3.1 (vs air)
vapor pressure   <1 mmHg ( 20 °C)
InChI Key   PEDCQBHIVMGVHV-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.5% (GC)
autoignition temp.   698 °F
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
ign. residue   ≤0.1%
refractive index   n20/D 1.474(lit.)
  n20/D 1.474
pH   5.5-8 (25 °C, 5 M in H2O)
bp   182 °C/20 mmHg(lit.)
mp   20 °C(lit.)
solubility   H2O: soluble5 M at 20 °C, clear, colorless
density   1.25 g/mL(lit.)
anion traces   chloride (Cl-): ≤1 mg/kg
  sulfate (SO42-): ≤10 mg/kg
cation traces   Ag: ≤5 mg/kg
  Al: ≤1 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤1 mg/kg
  Bi: ≤1 mg/kg
  Ca: ≤5 mg/kg
  Cd: ≤1 mg/kg
  Co: ≤1 mg/kg
  Cr: ≤1 mg/kg
  Cu: ≤1 mg/kg
  Fe: ≤1 mg/kg
  K: ≤20 mg/kg
  Li: ≤1 mg/kg
  Mg: ≤1 mg/kg
  Mn: ≤1 mg/kg
  Mo: ≤1 mg/kg
  NH4+: ≤5 mg/kg
  Na: ≤20 mg/kg
  Ni: ≤1 mg/kg
  Pb: ≤1 mg/kg
  Sr: ≤1 mg/kg
  Tl: ≤5 mg/kg
  Zn: ≤1 mg/kg
λ   5 M in H2O
UV absorption   λ: 260 nm Amax: 0.05
  λ: 280 nm Amax: 0.04

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Other Notes

Component of loading buffer in agarose gel electrophoresis of nucleic acids previously denatured with glyoxal; Preparation of phage and plasmid DNA, for the storage of pure cultures

Packaging

1 L in glass bottle

100, 250 mL in glass bottle

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.




Microcon Centrifugal Filters

All labs need water
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
MA8050000
Flash Point(F) 
320 °F
Flash Point(C) 
160 °C
Protocols & Articles

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References

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