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49767 Sigma

Glycerol

BioUltra, for molecular biology, anhydrous, ≥99.5% (GC)

Synonym: 1,2,3-Propanetriol, Glycerin

  • CAS Number 56-81-5

  • Linear Formula HOCH2CH(OH)CH2OH

  • Molecular Weight 92.09

  •  Beilstein Registry Number 635685

  •  EC Number 200-289-5

  •  MDL number MFCD00004722

  •  PubChem Substance ID 57651220

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Properties

Related Categories Alphabetical, BioUltra, Biochemicals and Reagents, Biostabilization Agents, Cell Biology,
grade   for molecular biology
vapor density   3.1 (vs air)
vapor pressure   <1 mmHg ( 20 °C)
InChI Key   PEDCQBHIVMGVHV-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.5% (GC)
autoignition temp.   698 °F
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
ign. residue   ≤0.1%
refractive index   n20/D 1.474(lit.)
  n20/D 1.474
pH   5.5-8 (25 °C, 5 M in H2O)
bp   182 °C/20 mmHg(lit.)
mp   20 °C(lit.)
solubility   H2O: soluble5 M at 20 °C, clear, colorless
density   1.25 g/mL(lit.)
anion traces   chloride (Cl-): ≤1 mg/kg
  sulfate (SO42-): ≤10 mg/kg
cation traces   Ag: ≤5 mg/kg
  Al: ≤1 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤1 mg/kg
  Bi: ≤1 mg/kg
  Ca: ≤5 mg/kg
  Cd: ≤1 mg/kg
  Co: ≤1 mg/kg
  Cr: ≤1 mg/kg
  Cu: ≤1 mg/kg
  Fe: ≤1 mg/kg
  K: ≤20 mg/kg
  Li: ≤1 mg/kg
  Mg: ≤1 mg/kg
  Mn: ≤1 mg/kg
  Mo: ≤1 mg/kg
  NH4+: ≤5 mg/kg
  Na: ≤20 mg/kg
  Ni: ≤1 mg/kg
  Pb: ≤1 mg/kg
  Sr: ≤1 mg/kg
  Tl: ≤5 mg/kg
  Zn: ≤1 mg/kg
λ   5 M in H2O
UV absorption   λ: 260 nm Amax: 0.05
  λ: 280 nm Amax: 0.04

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Other Notes

Component of loading buffer in agarose gel electrophoresis of nucleic acids previously denatured with glyoxal; Preparation of phage and plasmid DNA, for the storage of pure cultures

Packaging

1 L in glass bottle

100, 250 mL in glass bottle

Biochem/physiol Actions

Glycerol has been used as
• a supplement during cell culture of Mycobacterium tuberculosis and Mycobacterium avium.
• a fuel during designing enzymatic biofuel cell.
• a liquid composite matrix with 4-HCCA and 3-amino­quinoline for analysis of neutral and acidic glycans.
• a matrix for fast atom bombardment MS.
• may be employed as liquid matrix for the quantification studies by MALDI (Matrix-assisted laser desorption/ionization mass spectrometry) analysis.

Glycerol is hygroscopic in nature and is soluble in water owing to its three hydrophilic alcoholic hydroxyl groups. It can form both inter- and intramolecular hydrogen bonds, making it a very flexible molecule. The physiologic effect of glycerine is due to cell-mediated immunity, increased IgG production and increased histamine release.

General description

Glycerol is odourless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact to the environment.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.




Microcon Centrifugal Filters

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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
MA8050000
Flash Point(F) 
320 °F
Flash Point(C) 
160 °C
Protocols & Articles

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