Synonym: Atto 655 [goat Anti-mouse IgG]
|Related Categories||Alphabetical Index, Antibodies, Antibody Conjugates, Atto 655, Atto Antibody Conjugates,|
|concentration||~1 mg/mL IgG|
|fluorescence||λex 655 nm; λem 677 nm in PBS|
|shipped in||wet ice|
Unconjugated dye ≤5% of total fluorescence.
Anti-mouse IgG (whole molecule) (Sigma, M8645) is developed in goat using purified mouse IgG as the immunogen. Affinity isolated antigen specific antibody is purified from goat anti-mouse IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity. Goat anti-mouse is conjugated to Atto 655 NHS (Abs.max. 663 nm; Em.max. 684 nm),(Fluka 76245), then further purified via gel permeation chromatography and dialysis to remove unbound Atto-Dye.
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