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50933 Sigma

Guanidine hydrochloride

BioUltra, for molecular biology, ≥99.5% (AT)

Synonym: Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride

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Properties

Related Categories Alphabetical, BioUltra, Biochemicals and Reagents, Chaotropic Agents, DNA & RNA Purification,
grade   for molecular biology
InChI Key   PJJJBBJSCAKJQF-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.5% (AT)
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
ign. residue   ≤0.05% (as SO4)
loss   ≤0.05% loss on drying, 20 °C (HV)
pH   4.5-6.0 (25 °C, 6 M in H2O)
mp   180-185 °C(lit.)
solubility   H2O: soluble6 M at 20 °C, clear, colorless
anion traces   sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   6 M in H2O
UV absorption   λ: 260 nm Amax: 0.03
  λ: 280 nm Amax: 0.02

Description

Application

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Other Notes

Denaturant. Isolation of RNA

Price and Availability


Microcon Centrifugal Filters

All labs need water
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles
Peer-Reviewed Papers
15

References

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