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50980 Sigma

Guanidine thiocyanate

BioUltra, for molecular biology, ≥99.0% (AT)

Synonym: Guanidinium rhodanide, Guanidinium thiocyanate

  • CAS Number 593-84-0

  • Linear Formula NH2C(=NH)NH2 · HSCN

  • Molecular Weight 118.16

  •  Beilstein Registry Number 3563461

  •  EC Number 209-812-1

  •  MDL number MFCD00013027

  •  PubChem Substance ID 57651290

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Properties

Related Categories Alphabetical, BioUltra, Biochemicals and Reagents, Chaotropic Agents, DNA & RNA Purification,
grade   for molecular biology
InChI Key   ZJYYHGLJYGJLLN-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.0% (AT)
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
ign. residue   ≤0.5% (as SO4)
loss   ≤0.1% loss on drying, 20 °C (HV)
pH   4.5-7.0 (25 °C, 6 M in H2O)
mp   117-120 °C
  120-122 °C(lit.)
solubility   H2O: soluble6 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤5000 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤5000 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
absorption   absorption6M/280 nm, H2O cut-off

Description

Application

Chaotropic agent and strong denaturant; solubilizes cells.

Other Notes

Isolation of RNA; A new guanidine thiocyanate procedure for the isolation of full-length RNA templates for reverse transcription from tissues rich in RNase and proteoglycans

Price and Availability


Microcon Centrifugal Filters

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Supplemental Hazard Statements 
Contact with acids liberates very toxic gas.
Personal Protective Equipment 
RIDADR 
UN 1759 8 / PGIII
WGK Germany 
2
RTECS 
XL1225000

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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