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51456 Sigma

Urea

BioUltra, for molecular biology, ≥99.5% (T)

Synonym: Carbamide, Carbonyldiamide

  • CAS Number 57-13-6

  • Linear Formula NH2CONH2

  • Molecular Weight 60.06

  •  Beilstein Registry Number 635724

  •  EC Number 200-315-5

  •  MDL number MFCD00008022

  •  PubChem Substance ID 24873567

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Properties

Related Categories Alphabetical, BioUltra, Biochemicals and Reagents, Chaotropic Agents, DNA & RNA Purification,
grade   for molecular biology
InChI Key   XSQUKJJJFZCRTK-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.5% (T)
form   powder or crystals
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
  ≤0.1% biuret
  <0.005% formaldehyde (HCHO)
ign. residue   ≤0.01% (as SO4)
loss   ≤0.1% loss on drying, 20 °C (HV)
pH   8.0-10.0 (20 °C, 8 M in H2O)
mp   132-135 °C(lit.)
solubility   H2O: soluble8 M at 20 °C, clear, colorless
density   1.335 g/mL at 25 °C(lit.)
anion traces   chloride (Cl-): ≤5 mg/kg
  sulfate (SO42-): ≤10 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   8 M in H2O
UV absorption   λ: 260 nm Amax: ≤0.03
  λ: 280 nm Amax: ≤0.02
Gene Information   human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

Description

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Other Notes

Denaturing reagent; Commonly used in conjunction with polyacrylamide gel electrophoresis for the fractionation of single-stranded nucleic acids with chains of up to 200 nucleotides

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.




Microcon Centrifugal Filters

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
YR6250000
Protocols & Articles
Peer-Reviewed Papers
15

References

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