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53048 Sigma

5-Carboxy-tetramethylrhodamine N-succinimidyl ester

BioReagent, suitable for fluorescence

Synonym: "4-Carboxytetramethylrhodamine N-succinimidyl ester", 5-TAMRA

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Properties

Related Categories Biochemicals and Reagents, Detection, Fluorescent Labels, Fluorescent Probes, Labels, Particles and Stains, Fluorescent Stains,
InChI Key   VWFRSNKRTNUMET-UHFFFAOYSA-N
product line   BioReagent
solubility   DMF: soluble
  acetonitrile: soluble
fluorescence   λex 543 nm; λem 578 nm in 0.1 M phosphate pH 7.0
suitability   suitable for fluorescence
storage temp.   −20°C

Description

Other Notes

Fluorescent labeling of DNA and RNA

Application

5-Carboxy-tetramethylrhodamine N-succinimidyl ester (5-TAMRA) is used as an amine coupling reagent to form 5-carboxy-tetramethylrhodamine (5-TAMRA) derivatized compounds. 5-TAMRA derivatized compounds may be used in applications as enzyme substrates, molecular probes, molecular beacons and as fluorescence resonance energy transfer (FRET) partners.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Fluorescent Labeling of Peptides

Labeling peptides with fluorescent dyes or other labels provides powerful tools for the investigation of biological relevant interactions like receptor-ligand-binding,1-3 protein structures,4-6 and e...
ChemFiles Volume 5 Article 12
Keywords: Alzheimer Disease, Biochemistry, Electrophoresis, Enzyme activity, Immobilization, Ligands, Methods, Microscopy, Peptide synthesis, Separation, Solid phase peptide synthesis, Solvents, Spectroscopy, Tools

Peer-Reviewed Papers
15

References

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