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54457 Sigma

HEPES

BioUltra, for molecular biology, ≥99.5% (T)

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

  • CAS Number 7365-45-9

  • Empirical Formula (Hill Notation) C8H18N2O4S

  • Molecular Weight 238.30

  •  Beilstein Registry Number 883043

  •  EC Number 230-907-9

  •  MDL number MFCD00006158

  •  PubChem Substance ID 24878774

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Properties

Related Categories Alphabetical, BioUltra, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers,
grade   for molecular biology
InChI Key   JKMHFZQWWAIEOD-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.5% (T)
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
ign. residue   ≤0.1% (as SO4)
loss   ≤0.5% loss on drying, 110 °C
pH   5.0-6.5 (25 °C, 1 M in H2O)
useful pH range   6.8 - 8.2
pKa (25 °C)   7.5
solubility   H2O: soluble1 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   1 M in H2O
UV absorption   λ: 260 nm Amax: 0.045
  λ: 280 nm Amax: 0.035

Description

Other Notes

3′-Terminal labeling of RNA with T4 RNA ligase; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer.

Easily compare specifications for HEPES products with the HEPES specification table.

Price and Availability


Interactive Buffer Table
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TL6809000
Protocols & Articles

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