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55021C Sigma

Hanks’ Balanced Salt solution

HBSS Modified, with phenol red, without calcium, without magnesium, liquid



Related Categories Balanced Salt Mixtures and Solutions, Cell Culture, Classic Media and Salts, Hank’s Balanced Salts
Quality Level   GMP
description   for research or for further manufacturing use
form   liquid



Hanks’ Balanced Salt Solution (HBSS) is designed for use with cells maintained in non-CO2 atmospheric conditions. For cell dissociation procedures, use HBSS Modified, which is formulated without calcium and magnesium salts.

General description

The essential function of Hanks′ Balanced Salt solution is to maintain pH as well as osmotic balance. It also provides water and essential inorganic ions to cells.

Price and Availability

Polymeric Drug Delivery Techniques Guide

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Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture

With sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

10 ×, Modified, without calcium, magnesium or sodium bicarbonate

Safety & Documentation
Protocols & Articles
Peer-Reviewed Papers


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Current developments in interim transport (storage) media in dentistry: an update Malhotra N Br. Dent. J. 211, 29, (2011)


Morphological changes of porcine islets of Langerhans after collagenase and HBSS infusion of the pancreas Hilling DE Xenotransplantation 17, 413, (2010)


Viability of human periodontal ligament fibroblasts in milk, Hank's balanced salt solution and coconut water as storage media Souza BD Int. Endod. J. 44, 111, (2011)


Effect of HBSS storage time on human periodontal ligament fibroblast viability de Souza BD Dent. Traumatol. 26, 481, (2010)


Flagellin-dependent and -independent inflammatory responses following infection by enteropathogenic Escherichia coli and Citrobacter rodentium. Khan MA Infect. Immun. 76(4), 1410-22, (2008)


A critical role for Neurofascin in regulating action potential initiation through maintenance of the axon initial segment. Zonta B Neuron 69(5), 945-56, (2011)


Chitosan nanoparticles act as an adjuvant to promote both Th1 and Th2 immune responses induced by ovalbumin in mice. Zheng-Shun Wen et al Mar. Drugs 9, 1038-55, (2011)


Chronic antidiabetic sulfonylureas in vivo: reversible effects on mouse pancreatic beta-cells. Remedi MS PLoS Med. 5(10), e206, (2008)


A single-amino-acid substitution in a polymerase protein of an H5N1 influenza virus is associated with systemic infection and impaired T-cell activation in mice. Jamie L. Fornek et al J. Virol. 83, 11102-15, (2009)


A balanced diet is necessary for proper entrainment signals of the mouse liver clock. Akiko Hirao et al PLoS ONE 4, e6909, (2009)


Feeding cues and injected nutrients induce acute expression of multiple clock genes in the mouse liver. Oike H PLoS ONE 6(8), e23709, (2011)


Adhesion of Candida albicans to endothelial cells under physiological conditions of flow. Grubb SE Infect. Immun. 77(9), 3872-8, (2009)


Insulin receptor signaling regulates actin cytoskeletal organization in developing photoreceptors. Rajala RV J. Neurochem. 110(5), 1648-60, (2009)


Enhanced antitumor efficacy of cisplatin in combination with HemoHIM in tumor-bearing mice. Park HR BMC Cancer 9, 85, (2009)


Characterization of primary neurospheres generated from mouse ventral rostral hindbrain. Osterberg N Cell Tissue Res. 336(1), 11-20, (2009)


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