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56304 Sigma

Atto 565-Streptavidin

BioReagent, suitable for fluorescence

Synonym: Streptavidin-Atto 565



Related Categories Biochemicals and Reagents, Fluorescent Conjugates, Fluorescent Probes, Labels, Particles and Stains, Streptavidin/Avidin Conjugates
product line   BioReagent
fluorescence   λex 565 nm; λem 590 nm in 0.1 M phosphate pH 7.0
suitability   corresponds for gel electrophoresis
  suitable for fluorescence
storage temp.   −20°C


Analysis Note

free of unconjugated dye

General description

find more information here

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.


Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). It does nearly not show cis trans-isomerisation, which limits brightness and reproducibility for many other dyes. Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Streptavidin conjugation of Atto 565 does not significantly change the labels spectral data regarding excitation and emission maxima. Atto 565-Streptavidin is used to label biotin conjugated molecules.

Price and Availability

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Fluorescent Labeling of Peptides

Labeling peptides with fluorescent dyes or other labels provides powerful tools for the investigation of biological relevant interactions like receptor-ligand-binding,1-3 protein structures,4-6 and e...
ChemFiles Volume 5 Article 12
Keywords: Alzheimer Disease, Biochemistry, Electrophoresis, Enzyme activity, Immobilization, Ligands, Methods, Microscopy, Peptide synthesis, Separation, Solid phase peptide synthesis, Solvents, Spectroscopy, Tools

Superior Fluorescent Imaging Using Atto Dyes

Activated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescen...
BioFiles 2011, 6.3, 5.
Keywords: Absorption, Applications, Bacterial conjugations, Confocal microscopy, Degradations, Enzyme Assays, Extinction coefficient, Flow cytometry, In Situ hybridization, Infrared spectroscopy, Isomerizations, Microarray Analysis, Microscopy, Molecular probes, Nucleic acid hybridization, Protocols, Semiconductor, Separation, Spectroscopy, Support

Peer-Reviewed Papers


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