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56500 Sigma

GenElute Agarose Spin Columns

sufficient for 70 purifications

Synonym: DNA purification from agarose gel, Gen Elute

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Description

Other Notes

GenElute Agarose Spin Columns isolate DNA by centrifugation/filtration.

For additional information, please see www.sigma-aldrich.com/post-reaction.

Application

The purified DNA can be used in most downstream molecular biology applications including ligation, PCR, restriction digest, cloning, labeling and hybridization.

Features and Benefits

• One-step, 10 minute protocol
• No melting or digestion of agarose required
• Simpler and quicker than bind and elute or electroelution methods
• No ethanol precipitation
• Typical recovery of 40 to 45% for DNA fragments from 100 bp to 10 kb (Figs. 1 and 2)

Principle

GenElute Agarose Spin Columns are designed for the rapid purification of linear DNA (100 bp to 10 kb) from agarose gels. The DNA band is excised from an agarose gel and loaded into the spin column. Embedded within the base of the column are a series of membranes and filters that hold agarose and impurities back while allowing DNA to selectively pass through the column into a collection tube during a 10 minute centrifugation. GenElute Agarose Spin Columns eliminate the need for silica-based resins, DEAE or toxic organic solvents such as phenol and chloroform. There is no melting, electroelution or enzymatic digestion of the agarose gel.

General description

GenElute Agarose Spin Columns allow for the purification of linear DNA from agarose gels. The typical recovery is 40 to 45% for 100 bp to 10 kb DNA fragments. The recovery decreases as the fragment size increases.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

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Safety & Documentation

Safety Information

WGK Germany 
3
Protocols & Articles

Articles

Nancy-520 for DNA Detection and Quantitation

Nancy-520 is a fluorescent stain for double stranded DNA (dsDNA) on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is...
Monika Baeumle
BioFiles 2009, 4.1, 4.
Keywords: Catalytic combustion detector, Electrophoresis

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