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56748 Sigma

Imidazole

BioUltra, for molecular biology, ≥99.5% (GC)

Synonym: 1,3-Diaza-2,4-cyclopentadiene, Glyoxaline

  • CAS Number 288-32-4

  • Empirical Formula (Hill Notation) C3H4N2

  • Molecular Weight 68.08

  •  Beilstein Registry Number 103853

  •  EC Number 206-019-2

  •  MDL number MFCD00005183

  •  PubChem Substance ID 57651596

  •  eCl@ss 39161001

  • Popular Documents:  Datasheet (PDF)  

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Properties

Related Categories Alphabetical, BioUltra, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers,
grade   for molecular biology
vapor pressure   <1 mmHg ( 20 °C)
InChI Key   RAXXELZNTBOGNW-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.5% (GC)
form   flakes
  powder or crystals
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
  ≤0.2% water
ign. residue (900 °C)   ≤0.05%
pH   9.5-11.0 (25 °C, 0.1 M in H2O)
pKa (25 °C)   6.95
bp   256 °C(lit.)
mp   88-91 °C(lit.)
solubility   H2O: soluble0.1 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   0.1 M in H2O
UV absorption   λ: 260 nm Amax: 0.10
  λ: 280 nm Amax: 0.10

Description

Application

Excellent for buffers in the range of pH 6.2-7.8

Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for assay of horseradish peroxidase and as chelator for the binding of various divalent cations. Imidazole can be used for the elution of histidine containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for detection of proteins.

Other Notes

Review: As RNase model

Price and Availability


Interactive Buffer Table

Microcon Centrifugal Filters

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3263 8 / PGIII
WGK Germany 
2
RTECS 
NI3325000
Flash Point(F) 
293 °F
Flash Point(C) 
145 °C
Protocols & Articles

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Peer-Reviewed Papers
15

References

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