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58030 Sigma

Iodonitrotetrazolium chloride

BioReagent, ≥97.0% (calc. on dry substance, NT)

Synonym: 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT

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Properties

Related Categories Biochemicals and Reagents, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Histidinol Dehydrogenase, Ketolisomerase,
InChI Key   JORABGDXCIBAFL-UHFFFAOYSA-M
product line   BioReagent
assay   ≥97.0% (calc. on dry substance, NT)
mp   240 °C (dec.)(lit.)
  ~245 °C (dec.)
solubility   methanol: water (1:1): soluble50 mg/mL, very faintly turbid, very deep yellow (hot)

Description

Application

Electron acceptor for the colorimetric assay of various dehydrogenases

Iodonitrotetrazolium (INT) is a tetrazolium dye precursor that forms a purple formazan dye on reduction and has been used in a variety of applications. It is considered to have higher reactivity than some tetrazolium compounds, at least with respect to succinate dehydrogenase, with optimal results obtained using a concentration of 0.8 mM INT. INT is used as an electron acceptor for the colorimetric assays of: lactate dehydrogenase, xanthine dehydrogenase, lactyl-CoA dehydrogenase, succinate dehydrogenase, BBM II ketolisomerase, histidinol dehydrogenase and diverse other hydrolases.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

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