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60630 Sigma

Catalase from bovine liver

crystalline suspension in water, pH ~6, ~65000 U/mg protein (~20 mg/ml)

Synonym: H2O2:H2O2 oxidoreductase

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Properties

Related Categories 1.11.1.6 Catalase, 1.x.x.x Oxidoreductases, Application Index, Biochemicals and Reagents, Cell Biology,
quality   crystalline suspension in water, pH ~6
mol wt   tetramer mol wt ~250 kDa
  Mr ~240000
contains   ~0.01% alkylbenzyldimethyl ammoniumchloride as stabilizer
isoelectric point   5.4
storage temp.   2-8°C
Gene Information   cow ... CAT(280743)

Description

Components

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Application

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalses have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Biochem/physiol Actions

Catalase catalyzes the degradation of hydrogen peroxide into water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor.

Other Notes

Sales restrictions may apply

Structure; Properties; Used for analytical purposes, e.g. the determination of cysteamine; Hydrogen peroxide detection from activated phagocytes by a peroxidase-independent method;

Unit Definition

1 U corresponds to the amount of enzyme which decomposes 1 μmol H2O2 per minute at pH 7.0 and 25°C

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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