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62288 Sigma

Lipase B Candida antarctica, recombinant from Aspergillus oryzae

powder, beige, ~9 U/mg

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Lipase B from Candida antarctica has been shown to be an effective catalyst for the synthesis of esters of ethyl D-glucopyranoside from fatty acids larger than octanoic acid. It has also been found to catalyze a wide variety of organic reactions including many different regio- and enantio-selective syntheses.

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 8.0 and 40°C (tributyrin, Cat. No. 91010, as substrate)

Application

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

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Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TO9776500

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What solvent can Lipase B Candida antarctica, recombinant from Aspergillus oryzae, Product 62288, be dissolved in?
Lipase B is expected to be soluble in water. We are able to dissolve the enzyme in water for the enzymatic assay. We also have tested the solubility of Lipase B in 0.05M potassium phosphate buffer solution (pH=7.5) during quality control at a concentration of 10mg/mL. According to the Merck Index, almost all organic solvents decrease lipase activity, with the exception of petroleum ether.  
Is Product 62288, Lipase B Candida antarctica, recombinant from Aspergillus oryzae, lyophilized?
This lipase is a lyophilisate and may contain Gum arabic for adjustment of activity. The amount of Gum arabic in each vial is lot dependent.
How pure is Product 62288, Lipase B Candida antarctica, recombinant from Aspergillus oryzae?
Purity is not determined for this product. As is the case with many of our enzymes, the product is assayed for enzymatic activity, not for purity.
What is the molecular weight of Lipase B Candida antarctica, recombinant from Aspergillus oryzae, Product 62288?
Unfortunately, molecular weight information is unavailable at this time.
What is Product 62288, Lipase B Candida antarctica, recombinant from Aspergillus oryzae, soluble in?
Lipase B is expected to be soluble in water. We are able to dissolve the enzyme in water for the enzymatic assay. We also have tested the solubility of Lipase B in 0.05 M potassium phosphate buffer solution (pH=7.5) during quality control at a concentration of 10 mg/mL. According to the Merck Index, almost all organic solvents decrease lipase activity, with the exception of petroleum ether.
Where can I find more information about Product 62288, Lipase B Candida antarctica, recombinant from Aspergillus oryzae?
The BRENDA enzyme information system can be accessed by clicking on the "BRENDA" link on the product detail page (see link below). This database gives references and information on solubility, potential reactions with the enzyme, stability and applications. http://www.sigmaaldrich.com/catalog/search/ProductDetail/FLUKA/62288
Where can I find other enzymes, substrates, activators and inhibitors that Sigma-Aldrich offers?
Please visit our Enzyme Explorer tool at the following link: http://www.sigmaaldrich.com/Area_of_Interest/Biochemicals/Enzyme_Explorer.html
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Peer-Reviewed Papers
15

References

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