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63689 Sigma

2-Mercaptoethanol

BioUltra, for molecular biology, ≥99.0% (GC)

Synonym: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

  • CAS Number 60-24-2

  • Linear Formula HSCH2CH2OH

  • Molecular Weight 78.13

  •  Beilstein Registry Number 773648

  •  EC Number 200-464-6

  •  MDL number MFCD00004890

  •  PubChem Substance ID 57651953

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Properties

Related Categories BioUltra, Molecular Biology, Molecular Biology Reagents More...
grade   for molecular biology
vapor density   2.69 (vs air)
vapor pressure   1 mmHg ( 20 °C)
InChI Key   DGVVWUTYPXICAM-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.0% (GC)
expl. lim.   18 %
concentration   14.3 M (pure liquid)
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
refractive index   n20/D 1.500(lit.)
  n20/D 1.501
bp   157 °C(lit.)
solubility   H2O: 0.5 M at 20 °C clear, colorless
density   1.114 g/mL at 25 °C(lit.)
cation traces   Al: ≤0.5 mg/kg
  Ba: ≤0.1 mg/kg
  Bi: ≤0.1 mg/kg
  Ca: ≤0.5 mg/kg
  Cd: ≤0.05 mg/kg
  Co: ≤0.02 mg/kg
  Cr: ≤0.02 mg/kg
  Cu: ≤0.02 mg/kg
  Fe: ≤0.1 mg/kg
  K: ≤0.5 mg/kg
  Li: ≤0.1 mg/kg
  Mg: ≤0.1 mg/kg
  Mn: ≤0.02 mg/kg
  Mo: ≤0.1 mg/kg
  Na: ≤1 mg/kg
  Ni: ≤0.05 mg/kg
  Pb: ≤0.1 mg/kg
  Sr: ≤0.1 mg/kg
  Zn: ≤0.1 mg/kg
λ   0.5 M in H2O
UV absorption   λ: 260 nm Amax: 1.5
  λ: 280 nm Amax: 0.3
storage temp.   2-8°C

Description

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

2-Mercaptoethanol has been used
• in the protein extraction buffer prepared for leaf samples.
• as a supplement in cell culture media.
• in the procedure of RNA extraction.

Other Notes

Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

Signal word 
Danger
Target organs 
Heart, Liver
RIDADR 
UN 2966 6.1 / PGII
WGK Germany 
3
RTECS 
KL5600000
Flash Point(F) 
165.2 °F
Flash Point(C) 
74 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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