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63689 Sigma

2-Mercaptoethanol

BioUltra, for molecular biology, ≥99.0% (GC)

DOWNLOAD MSDS (PDF)

Synonym: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

  • CAS Number 60-24-2

  • Linear Formula HSCH2CH2OH

  • Molecular Weight 78.13

  •  Beilstein Registry Number 773648

  •  EC Number 200-464-6

  •  MDL number MFCD00004890

  •  PubChem Substance ID 24882813

2-Mercaptoethanol BioUltra, for molecular biology, ≥99.0% (GC)
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Properties

Related Categories BioUltra, DNA & RNA Purification, Molecular Biology, Molecular Biology Reagents, Reagents More...
grade   for molecular biology
vapor density   2.69 (vs air)
vapor pressure   1 mmHg ( 20 °C)
product line   BioUltra
assay   ≥99.0% (GC)
expl. lim.   18 %
concentration   14.3 M (pure liquid)
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
refractive index   n20/D 1.500(lit.)
  n20/D 1.501
bp   157 °C(lit.)
solubility   H2O: soluble0.5 M at 20 °C, clear, colorless
density   1.114 g/mL at 25 °C(lit.)
cation traces   Al: ≤0.5 mg/kg
  Ba: ≤0.1 mg/kg
  Bi: ≤0.1 mg/kg
  Ca: ≤0.5 mg/kg
  Cd: ≤0.05 mg/kg
  Co: ≤0.02 mg/kg
  Cr: ≤0.02 mg/kg
  Cu: ≤0.02 mg/kg
  Fe: ≤0.1 mg/kg
  K: ≤0.5 mg/kg
  Li: ≤0.1 mg/kg
  Mg: ≤0.1 mg/kg
  Mn: ≤0.02 mg/kg
  Mo: ≤0.1 mg/kg
  Na: ≤1 mg/kg
  Ni: ≤0.05 mg/kg
  Pb: ≤0.1 mg/kg
  Sr: ≤0.1 mg/kg
  Zn: ≤0.1 mg/kg
λ   0.5 M in H2O
UV absorption   λ: 260 nm Amax: 1.5
  λ: 280 nm Amax: 0.3
storage temp.   2-8°C
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Description

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Other Notes

Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds1

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