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65497 Sigma

TRIS acetate-EDTA Buffer solution

for Northern and Southern blotting, solution

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Properties

Related Categories Buffers, Molecular Biology, Protein Electrophoresis, Proteomics, SDS-PAGE More...
grade   for molecular biology
product line   BioReagent
form   solution
impurities   DNases, none detected
  RNases, none detected
  phosphatases, none detected
  proteases, none detected
pH   8.3±0.2
density   1.00 g/mL at 20 °C
storage temp.   room temp

Description

Components

The TAE liquid buffer is a 1x solution that contains 0.04M TRIS acetate and 0.001M EDTA in water.

Application

Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. Especially useful for dsDNA separations.

General description

TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but dsDNA runs faster with TAE buffer. This concentrate can be easily diluted to 0.5x before use (with molecular biology grade water).

Price and Availability

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Protocols & Articles

Articles

Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

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